PUBLICATION

Internal epitope tagging informed by relative lack of sequence conservation

Authors
Burg, L., Zhang, K., Bonawitz, T., Grajevskaja, V., Bellipanni, G., Waring, R., Balciunas, D.
ID
ZDB-PUB-161129-4
Date
2016
Source
Scientific Reports   6: 36986 (Journal)
Registered Authors
Balciunas, Darius, Bellipanni, Gianfranco
Keywords
CRISPR-Cas9 genome editing, Genetic engineering, Zebrafish
MeSH Terms
  • Amino Acid Sequence
  • Animals
  • Antibodies/genetics
  • Conserved Sequence/genetics*
  • Epitopes/genetics*
  • Proteins/genetics
  • Sequence Alignment/methods
  • Zebrafish
PubMed
27892520 Full text @ Sci. Rep.
Abstract
Many experimental techniques rely on specific recognition and stringent binding of proteins by antibodies. This can readily be achieved by introducing an epitope tag. We employed an approach that uses a relative lack of evolutionary conservation to inform epitope tag site selection, followed by integration of the tag-coding sequence into the endogenous locus in zebrafish. We demonstrate that an internal epitope tag is accessible for antibody binding, and that tagged proteins retain wild type function.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping