PUBLICATION
Using Touch-evoked Response and Locomotion Assays to Assess Muscle Performance and Function in Zebrafish
- Authors
- Sztal, T.E., Ruparelia, A.A., Williams, C., Bryson-Richardson, R.J.
- ID
- ZDB-PUB-161115-2
- Date
- 2016
- Source
- Journal of visualized experiments : JoVE (116): (Journal)
- Registered Authors
- Bryson-Richardson, Robert, Ruparelia, Avnika, Sztal, Tamar Esther
- Keywords
- none
- MeSH Terms
-
- Accelerometry/veterinary
- Animals
- Locomotion*
- Muscle Fibers, Fast-Twitch
- Muscle, Skeletal*
- Phenotype
- Swimming*
- Touch*
- Zebrafish
- PubMed
- 27842370 Full text @ J. Vis. Exp.
Citation
Sztal, T.E., Ruparelia, A.A., Williams, C., Bryson-Richardson, R.J. (2016) Using Touch-evoked Response and Locomotion Assays to Assess Muscle Performance and Function in Zebrafish. Journal of visualized experiments : JoVE. (116).
Abstract
Zebrafish muscle development is highly conserved with mammalian systems making them an excellent model to study muscle function and disease. Many myopathies affecting skeletal muscle function can be quickly and easily assessed in zebrafish over the first few days of embryogenesis. By 24 hr post-fertilization (hpf), wildtype zebrafish spontaneously contract their tail muscles and by 48 hpf, zebrafish exhibit controlled swimming behaviors. Reduction in the frequency of, or other alterations in, these movements may indicate a skeletal muscle dysfunction. To analyze swimming behavior and assess muscle performance in early zebrafish development, we utilize both touch-evoked escape response and locomotion assays. Touch-evoked escape response assays can be used to assess muscle performance during short burst movements resulting from contraction of fast-twitch muscle fibers. In response to an external stimulus, which in this case is a tap on the head, wildtype zebrafish at 2 days post-fertilization (dpf) typically exhibit a powerful burst swim, accompanied by sharp turns. Our method quantifies skeletal muscle function by measuring the maximum acceleration during a burst swimming motion, the acceleration being directly proportional to the force produced by muscle contraction. In contrast, locomotion assays during early zebrafish larval development are used to assess muscle performance during sustained periods of muscle activity. Using a tracking system to monitor swimming behavior, we obtain an automated calculation of the frequency of activity and distance in 6-day old zebrafish, reflective of their skeletal muscle function. Measurements of swimming performance are valuable for phenotypic assessment of disease models and high-throughput screening of mutations or chemical treatments affecting skeletal muscle function.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping