PUBLICATION

Effects of NDRG1 family proteins on photoreceptor outer segment morphology in zebrafish

Authors
Takita, S., Wada, Y., Kawamura, S.
ID
ZDB-PUB-161105-9
Date
2016
Source
Scientific Reports   6: 36590 (Journal)
Registered Authors
Takita, Shimpei
Keywords
Cell polarity, Cellular neuroscience
MeSH Terms
  • Animals
  • Animals, Genetically Modified/metabolism
  • Carps/metabolism
  • Cell Cycle Proteins/metabolism*
  • Cell Membrane/metabolism
  • Intracellular Signaling Peptides and Proteins/metabolism*
  • Photoreceptor Cells/metabolism*
  • Retina/metabolism
  • Retinal Cone Photoreceptor Cells/metabolism
  • Rod Cell Outer Segment/metabolism*
  • Zebrafish/metabolism*
(all 11)
PubMed
27811999 Full text @ Sci. Rep.
Abstract
Rods and cones are functionally and morphologically distinct. We previously identified N-myc downstream-regulated gene 1b (ndrg1b) in carp as a cone-specific gene. Here, we show that NDRG1b and its paralog, NDRG1a-1, contribute to photoreceptor outer segment (OS) formation in zebrafish. In adult zebrafish photoreceptors, NDRG1a-1 was localized in the entire cone plasma membranes, and also in rod plasma membranes except its OS. NDRG1b was expressed specifically in cones in the entire plasma membranes. In a developing retina, NDRG1a-1 was expressed in the photoreceptor layer, and NDRG1b in the photoreceptor layer plus inner nuclear layer. Based on our primary knockdown study suggesting that both proteins are involved in normal rod and cone OS development, NDRG1a-1 was overexpressed or NDRG1b was ectopically expressed in rods. These forced-expression studies in the transgenic fish confirmed the effect of these proteins on the OS morphology: rod OS morphology changed from cylindrical to tapered shape. These taper-shaped rod OSs were not stained with N,N'-didansyl cystine that effectively labels infolded membrane structure of cone OS. The result shows that rod OS membrane structure is preserved in these taper-shaped OSs and therefore, suggests that tapered OS morphology is not related to the infolded membrane structure in cone OS.
Genes / Markers
Marker Marker Type Name
gnat2GENEguanine nucleotide binding protein (G protein), alpha transducing activity polypeptide 2
ndrg1aGENEN-myc downstream regulated 1a
ndrg1bGENEN-myc downstream regulated 1b
ndrg3aGENEndrg family member 3a
opn1lw1GENEopsin 1 (cone pigments), long-wave-sensitive, 1
rhoGENErhodopsin
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Figures
Figure Gallery (6 images)
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Expression
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay Figure
gnat2TLstandard conditionsPrim-25RTPCR
gnat2TLstandard conditionsLong-pecRTPCR
gnat2TLstandard conditionsPec-finRTPCR
gnat2TLstandard conditionsPrim-5Not DetectedRTPCR
gnat2TLstandard conditionsDay 4RTPCR
gnat2TLstandard conditions5-9 somitesNot DetectedRTPCR
gnat2TLstandard conditions4-cellNot DetectedRTPCR
gnat2TLstandard conditionsProtruding-mouthRTPCR
ndrg1aTLstandard conditionsPrim-25RTPCR
ndrg1aTLstandard conditionsPrim-5RTPCR
1 - 10 of 34
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Phenotype
Fish Conditions Stage Phenotype Figure
TL + MO1-ndrg1astandard conditionsDay 4
TL + MO1-ndrg1astandard conditionsDay 4
zf781Tgstandard conditionsAdult
zf781Tgstandard conditionsAdult
zf782Tgstandard conditionsAdult
zf782Tgstandard conditionsAdult
zf783Tgstandard conditionsAdult
zf783Tgstandard conditionsAdult
1 - 8 of 8
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Mutations / Transgenics
Allele Construct Type Affected Genomic Region
zf780TgTransgenic Insertion
    zf781TgTransgenic Insertion
      zf782TgTransgenic Insertion
        zf783TgTransgenic Insertion
          1 - 4 of 4
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          Human Disease / Model
          No data available
          Sequence Targeting Reagents
          Target Reagent Reagent Type
          ndrg1aMO1-ndrg1aMRPHLNO
          1 - 1 of 1
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          Fish
          Fish
          TL + MO1-ndrg1a
          zf781Tg
          zf782Tg
          zf783Tg
          1 - 4 of 4
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          Antibodies
          Orthology
          No data available
          Engineered Foreign Genes
          Marker Marker Type Name
          GFPEFGGFP
          mCherryEFGmCherry
          1 - 2 of 2
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          Mapping
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          Citation
          Takita, S., Wada, Y., Kawamura, S. (2016) Effects of NDRG1 family proteins on photoreceptor outer segment morphology in zebrafish. Scientific Reports. 6:36590.