PUBLICATION
miR-19b Regulates Ventricular Action Potential Duration in Zebrafish
- Authors
- Benz, A., Kossack, M., Auth, D., Seyler, C., Zitron, E., Juergensen, L., Katus, H.A., Hassel, D.
- ID
- ZDB-PUB-161103-6
- Date
- 2016
- Source
- Scientific Reports 6: 36033 (Journal)
- Registered Authors
- Hassel, David
- Keywords
- Cardiovascular biology, Molecular biology
- MeSH Terms
-
- Action Potentials/genetics*
- Animals
- Arrhythmias, Cardiac/genetics*
- Arrhythmias, Cardiac/physiopathology
- Disease Models, Animal
- Heart Ventricles/physiopathology
- Humans
- Long QT Syndrome/genetics*
- Long QT Syndrome/physiopathology
- MicroRNAs/genetics*
- Myocardial Contraction/genetics
- Potassium Channels/genetics
- Zebrafish/genetics
- Zebrafish/physiology
- PubMed
- 27805004 Full text @ Sci. Rep.
Citation
Benz, A., Kossack, M., Auth, D., Seyler, C., Zitron, E., Juergensen, L., Katus, H.A., Hassel, D. (2016) miR-19b Regulates Ventricular Action Potential Duration in Zebrafish. Scientific Reports. 6:36033.
Abstract
Sudden cardiac death due to ventricular arrhythmias often caused by action potential duration (APD) prolongation is a common mode of death in heart failure (HF). microRNAs, noncoding RNAs that fine tune gene expression, are frequently dysregulated during HF, suggesting a potential involvement in the electrical remodeling process accompanying HF progression. Here, we identified miR-19b as an important regulator of heart function. Zebrafish lacking miR-19b developed severe bradycardia and reduced cardiac contractility. miR-19b deficient fish displayed increased sensitivity to AV-block, a characteristic feature of long QT syndrome in zebrafish. Patch clamp experiments from whole hearts showed that miR-19b deficient zebrafish exhibit significantly prolonged ventricular APD caused by impaired repolarization. We found that miR-19b directly and indirectly regulates the expression of crucial modulatory subunits of cardiac ion channels, and thereby modulates AP duration and shape. Interestingly, miR-19b knockdown mediated APD prolongation can rescue a genetically induced short QT phenotype. Thus, miR-19b might represent a crucial modifier of the cardiac electrical activity, and our work establishes miR-19b as a potential candidate for human long QT syndrome.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping