PUBLICATION
Evolution and Expression of Paxillin Genes in Teleost Fish
- Authors
- Jacob, A.E., Turner, C.E., Amack, J.D.
- ID
- ZDB-PUB-161103-4
- Date
- 2016
- Source
- PLoS One 11: e0165266 (Journal)
- Registered Authors
- Amack, Jeffrey
- Keywords
- Zebrafish, Embryos, Evolutionary genetics, Chromosomes, Genomic databases, Sequence motif analysis, Vertebrates, Phylogenetic analysis
- MeSH Terms
-
- Animals
- Evolution, Molecular
- Fish Proteins/genetics
- Fish Proteins/metabolism
- Fishes/embryology*
- Fishes/genetics
- Fishes/metabolism
- Focal Adhesions/metabolism*
- Gene Duplication
- Gene Expression Regulation, Developmental
- Humans
- Paxillin/genetics*
- Paxillin/metabolism*
- Phylogeny
- Synteny
- Zebrafish/embryology
- Zebrafish/genetics
- Zebrafish Proteins/genetics
- PubMed
- 27806088 Full text @ PLoS One
Citation
Jacob, A.E., Turner, C.E., Amack, J.D. (2016) Evolution and Expression of Paxillin Genes in Teleost Fish. PLoS One. 11:e0165266.
Abstract
Background Paxillin family proteins regulate intracellular signaling downstream of extracellular matrix adhesion. Tissue expression patterns and cellular functions of Paxillin proteins during embryo development remain poorly understood. Additionally, the evolution of this gene family has not been thoroughly investigated.
Results This report characterizes the evolution and expression of a novel Paxillin gene, called Paxillin-b, in Teleosts. Alignments indicate that Teleost Paxillin-a and Paxillin-b proteins are highly homologous to each other and to human Paxillin. Phylogenetic and synteny analyses suggest that these genes originated from the duplication of an ancestral Paxillin gene that was in a common ancestor of Teleosts and Tetrapods. Analysis of the spatiotemporal expression profiles of Paxillin-a and Paxillin-b using zebrafish revealed both overlapping and distinct domains for Paxillin-a and Paxillin-b during embryo development. Localization of zebrafish Paxillin orthologs expressed in mammalian cells demonstrated that both proteins localize to focal adhesions, similar to mammalian Paxillin. This suggests these proteins regulate adhesion-dependent processes in their endogenous tissues.
Conclusion Paxillin-a and Paxillin-b were generated by duplication in Teleosts. These genes likely play similar roles as Paxillin genes in other organisms. This work provides a framework for functional investigation of Paxillin family members during development using the zebrafish as an in vivo model system.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping