PUBLICATION
Innate Color Preference of Zebrafish and Its Use in Behavioral Analyses
- Authors
- Park, J.S., Ryu, J.H., Choi, T.I., Bae, Y.K., Lee, S., Kang, H.J., Kim, C.H.
- ID
- ZDB-PUB-161102-1
- Date
- 2016
- Source
- Molecules and cells 39(10): 750-755 (Journal)
- Registered Authors
- Bae, Young Ki, Kim, Cheol-Hee
- Keywords
- none
- MeSH Terms
-
- Animals
- Applied Behavior Analysis/methods*
- Behavior, Animal/physiology*
- Color*
- Female
- Male
- Zebrafish/physiology*
- PubMed
- 27802373 Full text @ Mol. Cells
Citation
Park, J.S., Ryu, J.H., Choi, T.I., Bae, Y.K., Lee, S., Kang, H.J., Kim, C.H. (2016) Innate Color Preference of Zebrafish and Its Use in Behavioral Analyses. Molecules and cells. 39(10):750-755.
Abstract
Although innate color preference of motile organisms may provide clues to behavioral biases, it has remained a longstanding question. In this study, we investigated innate color preference of zebrafish larvae. A cross maze with different color sleeves around each arm was used for the color preference test (R; red, G; green, B; blue, Y; yellow). The findings showed that 5 dpf zebrafish larvae preferred blue over other colors (B > R > G > Y). To study innate color recognition further, tyrosinase mutants were generated using CRISPR/Cas9 system. As a model for oculocutaneous albinism (OCA) and color vision impairment, tyrosinase mutants demonstrated diminished color sensation, indicated mainly by hypopigmentation of the retinal pigment epithelium (RPE). Due to its relative simplicity and ease, color preference screening using zebrafish larvae is suitable for high-throughput screening applications. This system may potentially be applied to the analysis of drug effects on larval behavior or the detection of sensory deficits in neurological disorder models, such as autism-related disorders, using mutant larvae generated by the CRISPR/Cas9 technique.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping