PUBLICATION
Advances in the Study of Heart Development and Disease Using Zebrafish
- Authors
- Brown, D.R., Samsa, L.A., Qian, L., Liu, J.
- ID
- ZDB-PUB-160624-5
- Date
- 2016
- Source
- Journal of cardiovascular development and disease 3(2): (Review)
- Registered Authors
- Brown, Daniel, Liu, Jiandong, Samsa, Leigh Ann
- Keywords
- cardiac arrhythmia, cardiomyopathy, congenital heart disease, development, drug screens, translational medicine, zebrafish
- MeSH Terms
- none
- PubMed
- 27335817 Full text @ J Cardiovasc Dev Dis
Citation
Brown, D.R., Samsa, L.A., Qian, L., Liu, J. (2016) Advances in the Study of Heart Development and Disease Using Zebrafish. Journal of cardiovascular development and disease. 3(2).
Abstract
Animal models of cardiovascular disease are key players in the translational medicine pipeline used to define the conserved genetic and molecular basis of disease. Congenital heart diseases (CHDs) are the most common type of human birth defect and feature structural abnormalities that arise during cardiac development and maturation. The zebrafish, Danio rerio, is a valuable vertebrate model organism, offering advantages over traditional mammalian models. These advantages include the rapid, stereotyped and external development of transparent embryos produced in large numbers from inexpensively housed adults, vast capacity for genetic manipulation, and amenability to high-throughput screening. With the help of modern genetics and a sequenced genome, zebrafish have led to insights in cardiovascular diseases ranging from CHDs to arrhythmia and cardiomyopathy. Here, we discuss the utility of zebrafish as a model system and summarize zebrafish cardiac morphogenesis with emphasis on parallels to human heart diseases. Additionally, we discuss the specific tools and experimental platforms utilized in the zebrafish model including forward screens, functional characterization of candidate genes, and high throughput applications.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping