PUBLICATION
A zebrafish intelectin ortholog agglutinates both Gram-negative and Gram-positive bacteria with binding capacity to bacterial polysaccharide
- Authors
- Chen, L., Yan, J., Sun, W., Sui, C., Qi, J., Du, Y., Feng, L.
- ID
- ZDB-PUB-160623-7
- Date
- 2016
- Source
- Fish & shellfish immunology 55: 729-36 (Journal)
- Registered Authors
- Feng, Lijun
- Keywords
- Bacterial agglutination, Innate immunity, LPS/PGN, Zebrafish intelectin
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Escherichia coli/physiology
- Escherichia coli Infections/genetics
- Escherichia coli Infections/immunology
- Escherichia coli Infections/metabolism
- Escherichia coli Infections/veterinary*
- Fish Diseases/genetics*
- Fish Diseases/immunology
- Fish Diseases/metabolism
- Lectins/chemistry
- Lectins/genetics*
- Lectins/metabolism
- Lipopolysaccharides/metabolism
- Peptidoglycan/metabolism
- Sequence Alignment/veterinary
- Staphylococcal Infections/genetics
- Staphylococcal Infections/immunology
- Staphylococcal Infections/metabolism
- Staphylococcal Infections/veterinary*
- Staphylococcus aureus/physiology
- Zebrafish*
- Zebrafish Proteins/chemistry
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/metabolism
- PubMed
- 27329687 Full text @ Fish Shellfish Immunol.
Citation
Chen, L., Yan, J., Sun, W., Sui, C., Qi, J., Du, Y., Feng, L. (2016) A zebrafish intelectin ortholog agglutinates both Gram-negative and Gram-positive bacteria with binding capacity to bacterial polysaccharide. Fish & shellfish immunology. 55:729-36.
Abstract
Intelectins are glycan-binding lectins found in various species including cephalochordates, urochordates, fish, amphibians and mammals. But their detailed functions are not well studied in zebrafish which is a good model to study native immunity. In this study, we cloned a zebrafish intelectin ortholog, zebrafish intelectin 2 (zITLN2), which contains a conserved fibrinogen-related domain (FReD) in the N-terminus and the unique intelectin domain in the C-terminus. We examined the tissue distribution of zITLN2 in adult zebrafish and found that zITLN2 was expressed in various organs with the highest level in intestine. Like amphioxus intelectins, zITLN2 expression was upregulated in adult zebrafish infected with Staphylococcus aureus with the highest expression level at 12 h after challenge. Recombinant zITLN2 protein expressed in E. coli was able to agglutinate both Gram-negative and Gram-positive bacteria to similar degrees in a calcium-dependent manner. Furthermore, recombinant zITLN2 bound lipopolysaccharide (LPS) and peptidoglycan (PGN) comparably. Our work on zITLN2 provided further information to understand functions of this new family of lectins and the innate immunity in vertebrates.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping