PUBLICATION

MARCKS-like protein is an initiating molecule in axolotl appendage regeneration

Authors
Sugiura, T., Wang, H., Barsacchi, R., Simon, A., Tanaka, E.M.
ID
ZDB-PUB-160406-10
Date
2016
Source
Nature   531: 237-40 (Journal)
Registered Authors
Keywords
Regeneration, Cell proliferation
MeSH Terms
  • Ambystoma mexicanum/injuries
  • Ambystoma mexicanum/physiology*
  • Amputation, Traumatic/metabolism
  • Animals
  • Cell Cycle/genetics
  • Cell Proliferation/genetics
  • Cloning, Molecular
  • Extremities/injuries
  • Extremities/physiology*
  • Humans
  • Intracellular Signaling Peptides and Proteins/genetics
  • Intracellular Signaling Peptides and Proteins/metabolism*
  • Membrane Proteins/genetics
  • Membrane Proteins/metabolism*
  • Mice
  • Molecular Sequence Data
  • Muscle Fibers, Skeletal/cytology
  • Muscle Fibers, Skeletal/physiology
  • Notophthalmus viridescens/genetics
  • Notophthalmus viridescens/injuries
  • Notophthalmus viridescens/physiology
  • Regeneration/physiology*
  • Tail/cytology
  • Tail/injuries
  • Tail/physiology
  • Wound Healing/physiology
  • Xenopus
  • Zebrafish
PubMed
26934225 Full text @ Nature
Abstract
Identifying key molecules that launch regeneration has been a long-sought goal. Multiple regenerative animals show an initial wound-associated proliferative response that transits into sustained proliferation if a considerable portion of the body part has been removed. In the axolotl, appendage amputation initiates a round of wound-associated cell cycle induction followed by continued proliferation that is dependent on nerve-derived signals. A wound-associated molecule that triggers the initial proliferative response to launch regeneration has remained obscure. Here, using an expression cloning strategy followed by in vivo gain- and loss-of-function assays, we identified axolotl MARCKS-like protein (MLP) as an extracellularly released factor that induces the initial cell cycle response during axolotl appendage regeneration. The identification of a regeneration-initiating molecule opens the possibility of understanding how to elicit regeneration in other animals.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping