PUBLICATION
MARCKS-like protein is an initiating molecule in axolotl appendage regeneration
- Authors
- Sugiura, T., Wang, H., Barsacchi, R., Simon, A., Tanaka, E.M.
- ID
- ZDB-PUB-160406-10
- Date
- 2016
- Source
- Nature 531: 237-40 (Journal)
- Registered Authors
- Keywords
- Regeneration, Cell proliferation
- MeSH Terms
-
- Ambystoma mexicanum/injuries
- Ambystoma mexicanum/physiology*
- Amputation, Traumatic/metabolism
- Animals
- Cell Cycle/genetics
- PubMed
- 26934225 Full text @ Nature
Abstract
Identifying key molecules that launch regeneration has been a long-sought goal. Multiple regenerative animals show an initial wound-associated proliferative response that transits into sustained proliferation if a considerable portion of the body part has been removed. In the axolotl, appendage amputation initiates a round of wound-associated cell cycle induction followed by continued proliferation that is dependent on nerve-derived signals. A wound-associated molecule that triggers the initial proliferative response to launch regeneration has remained obscure. Here, using an expression cloning strategy followed by in vivo gain- and loss-of-function assays, we identified axolotl MARCKS-like protein (MLP) as an extracellularly released factor that induces the initial cell cycle response during axolotl appendage regeneration. The identification of a regeneration-initiating molecule opens the possibility of understanding how to elicit regeneration in other animals.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping