PUBLICATION

Clarification of mural cell coverage of vascular endothelial cells by live imaging of zebrafish

Authors
Ando, K., Fukuhara, S., Izumi, N., Nakajima, H., Fukui, H., Kelsh, R.N., Mochizuki, N.
ID
ZDB-PUB-160310-12
Date
2016
Source
Development (Cambridge, England)   143(8): 1328-39 (Journal)
Registered Authors
Fukuhara, Shigetomo, Fukui, Hajime, Kelsh, Robert, Mochizuki, Naoki, Nakajima, Hiroyuki
Keywords
Mural cells, Pericytes, Vascular smooth muscle cells, Zebrafish, Pdgfrb
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Blood Vessels/cytology
  • Blood Vessels/embryology
  • Endothelial Cells/cytology*
  • Microscopy, Confocal
  • Muscle, Smooth, Vascular/cytology*
  • Pericytes/cytology*
  • Zebrafish
(all 9)
PubMed
26952986 Full text @ Development
Abstract
Mural cells (MCs) consisting of vascular smooth muscle cells and pericytes cover the endothelial cells (ECs) to regulate vascular stability and homeostasis. Here, we clarified the mechanism how MCs develop and cover ECs by generating the transgenic zebrafish lines that allow live imaging of MCs and by lineage tracing in vivo. To cover cranial vessels, MCs derived from either neural crest cells or mesoderm emerged around the preformed EC tubes, proliferated and migrated along EC tubes. During their migration, the MCs moved forward by extending their processes along the inter-EC junctions, suggesting the role of inter-EC junctions as a scaffold for MC migration. In the trunk vasculature, MCs derived from mesoderm covered the ventral side of the dorsal aorta (DA), but not the posterior cardinal vein. Furthermore, the MCs migrating from the DA or emerging around intersegmental vessels (ISV) preferentially covered arterial ISVs rather than venous ISVs, indicating that MCs mostly cover arteries during vascular development. Thus, live imaging and lineage tracing enabled us to precisely clarify how MCs cover the EC tubes and to identify the origins of MCs.
Genes / Markers
Marker Marker Type Name
cspg4GENEchondroitin sulfate proteoglycan 4
fabp2GENEfatty acid binding protein 2, intestinal
fli1GENEFli-1 proto-oncogene, ETS transcription factor
flt1GENEfms related receptor tyrosine kinase 1
foxd3GENEforkhead box D3
gmnnGENEgeminin DNA replication inhibitor
hand2GENEheart and neural crest derivatives expressed 2
pdgfrbGENEplatelet-derived growth factor receptor, beta polypeptide
pecam1aGENEplatelet and endothelial cell adhesion molecule 1a
taglnGENEtransgelin
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Figures
Figure Gallery (14 images) / 2
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Expression
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay Figure
EGFPncv1Tg; ncv22Tgstandard conditionsProtruding-mouthIFL
EGFPncv1Tg; ncv22Tgstandard conditionsDay 5IFL
EGFPncv1Tg; ncv22Tgstandard conditionsProtruding-mouthIFL
EGFPncv1Tg; ncv22Tgstandard conditionsLong-pec to Pec-finIFL
EGFPncv1Tg; ncv22Tgstandard conditionsDay 5IFL
EGFPncv1Tg; ncv22Tgstandard conditionsPrim-15 to Prim-25IFL
EGFPncv1Tg; ncv22Tgstandard conditionsDays 30-44IHC
EGFPncv1Tg; ncv22Tgstandard conditionsDays 30-44IHC
EGFPncv1Tg; ncv22Tgstandard conditionsPec-fin to Protruding-mouthIFL
EGFPncv1Tg; ncv22Tgstandard conditionsPec-fin to Protruding-mouthIFL
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Phenotype
Fish Conditions Stage Phenotype Figure
pdgfrbsa16389/sa16389; ncv1Tg; ncv22Tgstandard conditionsDay 5
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Mutations / Transgenics
Allele Construct Type Affected Genomic Region
ba74TgTransgenic Insertion
    ncv1TgTransgenic Insertion
      ncv2TgTransgenic Insertion
        ncv22TgTransgenic Insertion
          ncv23TgTransgenic Insertion
            ncv24TgTransgenic Insertion
              ncv25TgTransgenic Insertion
                ncv26TgTransgenic Insertion
                  ncv27TgTransgenic Insertion
                    ncv28TgTransgenic Insertion
                      1 - 10 of 17
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                      Human Disease / Model
                      No data available
                      Sequence Targeting Reagents
                      Target Reagent Reagent Type
                      foxd3MO1-foxd3MRPHLNO
                      hand2MO2-hand2MRPHLNO
                      tbx6MO1-tbx6MRPHLNO
                      tfap2aMO3-tfap2aMRPHLNO
                      tnnt2aMO1-tnnt2aMRPHLNO
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                      Fish
                      Fish
                      ba74Tg; ncv24Tg; ncv28Tg
                      ba74Tg; ncv24Tg; ncv28Tg; ncv29Tg
                      ncv1Tg; ncv22Tg
                      ncv1Tg; ncv24Tg; nkuasgfp1aTg
                      ncv1Tg; ncv25Tg
                      ncv22Tg
                      ncv23Tg
                      ncv23Tg; ncv2Tg
                      ncv23Tg; ncv27Tg
                      ncv24Tg; ncv26Tg
                      1 - 10 of 16
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                      Antibodies
                      Orthology
                      Engineered Foreign Genes
                      Marker Marker Type Name
                      CreEFGCre
                      DsRedEFGDsRed
                      EGFPEFGEGFP
                      GAL4FFEFGGAL4FF
                      mCherryEFGmCherry
                      RFPEFGRFP
                      VenusEFGVenus
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                      Mapping
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                      Citation
                      Ando, K., Fukuhara, S., Izumi, N., Nakajima, H., Fukui, H., Kelsh, R.N., Mochizuki, N. (2016) Clarification of mural cell coverage of vascular endothelial cells by live imaging of zebrafish. Development (Cambridge, England). 143(8):1328-39.