PUBLICATION

Characterization of the enhancer element of the Danio rerio minor globin gene locus

Authors
Nefedochkina, A.V., Petrova, N.V., Ioudinkova, E.S., Kovina, A.P., Iarovaia, O.V., Razin, S.V.
ID
ZDB-PUB-160206-3
Date
2016
Source
Histochemistry and cell biology   145(4): 463-73 (Journal)
Registered Authors
Keywords
Chromatin, Enhancer, Globin genes, Promoter, Zebrafish
MeSH Terms
  • Animals
  • Binding Sites
  • Cells, Cultured
  • Chick Embryo
  • Chickens
  • Enhancer Elements, Genetic/genetics*
  • Zebrafish/genetics*
  • alpha-Globins/genetics*
  • beta-Globins/genetics*
PubMed
26847176 Full text @ Histochem. Cell Biol.
Abstract
In Danio rerio, the alpha- and beta-globin genes are present in two clusters: a major cluster located on chromosome 3 and a minor cluster located on chromosome 12. In contrast to the segregated alpha- and beta-globin gene domains of warm-blooded animals, in Danio rerio, each cluster contains both alpha- and beta-globin genes. Expression of globin genes present in the major cluster is controlled by an erythroid-specific enhancer similar to the major regulatory element of mammalian and avian alpha-globin gene domains. The enhancer controlling expression of the globin genes present in the minor locus has not been identified yet. Based on the distribution of epigenetic marks, we have selected two genomic regions that might harbor an enhancer of the minor locus. Using transient transfection of constructs with a reporter gene, we have demonstrated that a ~500-bp DNA fragment located ~1.7 Kb upstream of the αe4 gene possesses an erythroid-specific enhancer active with respect to promoters present in both the major and the minor globin gene loci of Danio rerio. The identified enhancer element harbors clustered binding sites for GATA-1, NF-E2, and EKLF similar to the enhancer of the major globin locus on chromosome 3. Both enhancers appear to have emerged as a result of independent evolution of a duplicated regulatory element present in an ancestral single alpha-/beta-globin locus that existed before teleost-specific genome duplication.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping