PUBLICATION

Effects of tris (2-butoxyethyl) phosphate (TBOEP) on endocrine axes during development of early life stages of zebrafish (Danio rerio)

Authors
Ma, Z., Tang, S., Su, G., Miao, Y., Liu, H., Xie, Y., Giesy, J.P., Saunders, D.M., Hecker, M., Yu, H.
ID
ZDB-PUB-151108-1
Date
2016
Source
Chemosphere   144: 1920-1927 (Journal)
Registered Authors
Keywords
Endocrine axes, Endocrine disruptor, Environmentally relevant, Organophosphate flame retardant, qRT-PCR
MeSH Terms
  • Animals
  • Endocrine System/drug effects*
  • Endocrine System/physiology
  • Larva/drug effects
  • Larva/growth & development
  • Lethal Dose 50
  • Life Cycle Stages/drug effects*
  • Morphogenesis/drug effects
  • Organophosphorus Compounds/toxicity*
  • Time Factors
  • Water Pollutants, Chemical/toxicity*
  • Zebrafish/growth & development*
  • Zebrafish/metabolism
(all 13)
PubMed
26547027 Full text @ Chemosphere
CTD
26547027
Abstract
Due to phasing out of additive flame retardants such as polybrominated diphenyl ethers (PBDEs), Tris (2-butoxyethyl) phosphate (TBOEP) is widely used as a substitute. TBOEP is ubiquitous in the environment and has been measured at concentrations of micrograms per liter (μg L(-1)) in surface waters and wastewater. Information on potential adverse effects on development of aquatic organisms caused by exposure to environmentally relevant concentrations of TBOEP is limited, especially for effects that may be caused through impairment of endocrine-modulated homeostasis. Therefore, this study was conducted to determine effects of TBOEP on ontogeny and transcription profiles of genes along the hypothalamus-pituitary-thyroidal (HPT), hypothalamus-pituitary-adrenal (HPA), and hypothalamus-pituitary-gonadal (HPG) axes in embryos/larvae of zebrafish (Danio rerio). Exposure to TBOEP (2-5,000 μg L(-1)) from 3 h post-fertilization (hpf) to 120 hpf induced developmental malformations in zebrafish with a LC50 of 288.54 μg L(-1) at both 96 hpf and 120 hpf. The predicted no observed effect concentration (PNOEC) was 2.40 μg L(-1). Exposure to 2, 20, or 200 μg TBOEP L(-1) altered expression of genes involved in three major molecular pathways in a concentration-dependent manner after 120 hpf. TBOEP caused lesser expression of some genes involved in synthesis of hormones, such as (pomc and fshβ) as well as upregulating expression of some genes coding for receptors (thr, tshr, gr, mr, er and ar) in zebrafish larvae. These changes at the molecular level could result in alterations of endocrine function, which could result in edema or deformity and ultimately death.
Genes / Markers
Marker Marker Type Name
arGENEandrogen receptor
crhbGENEcorticotropin releasing hormone b
crhbpGENEcorticotropin releasing hormone binding protein
cyp11a1.1GENEcytochrome P450 family 11 subfamily A member 1, tandem duplicate 1
cyp19a1bGENEcytochrome P450, family 19, subfamily A, polypeptide 1b
dio1GENEiodothyronine deiodinase 1
dio2GENEiodothyronine deiodinase 2
esr1GENEestrogen receptor 1
esr2bGENEestrogen receptor 2b
fshbGENEfollicle stimulating hormone subunit beta
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Figures
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Expression
Phenotype
Fish Conditions Stage Phenotype Figure
ABchemical treatment: tris(2-butoxyethyl) phosphateProtruding-mouth
ABchemical treatment: tris(2-butoxyethyl) phosphateProtruding-mouth
ABchemical treatment: tris(2-butoxyethyl) phosphateDay 5
ABchemical treatment: tris(2-butoxyethyl) phosphateDay 5
ABchemical treatment: tris(2-butoxyethyl) phosphateDay 5
ABchemical treatment: tris(2-butoxyethyl) phosphateDay 5
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Mutations / Transgenics
No data available
Human Disease / Model
No data available
Sequence Targeting Reagents
No data available
Fish
Fish
AB
1 - 1 of 1
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Antibodies
No data available
Orthology
No data available
Engineered Foreign Genes
No data available
Mapping
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Citation
Ma, Z., Tang, S., Su, G., Miao, Y., Liu, H., Xie, Y., Giesy, J.P., Saunders, D.M., Hecker, M., Yu, H. (2016) Effects of tris (2-butoxyethyl) phosphate (TBOEP) on endocrine axes during development of early life stages of zebrafish (Danio rerio). Chemosphere. 144:1920-1927.