PUBLICATION
Expression and functionality of transient receptor potential melastatin 4 (TRPM4)-like channels during development of the zebrafish
- Authors
- Cheng, H., Ellis, J., Kleinow, K.M.
- ID
- ZDB-PUB-151004-5
- Date
- 2015
- Source
- Comparative biochemistry and physiology. Toxicology & pharmacology : CBP 178: 33-44 (Journal)
- Registered Authors
- Keywords
- Ca(2+), TRPM4, embryonic development, gene-expression, patch-clamp, zebrafish
- MeSH Terms
-
- Animals
- Calcium/metabolism
- Cell Differentiation/physiology
- Embryonic Development/physiology*
- Female
- Humans
- Male
- Membrane Potentials/physiology
- TRPM Cation Channels/metabolism*
- Zebrafish/embryology*
- Zebrafish/metabolism
- PubMed
- 26432160 Full text @ Comp. Biochem. Physiol. C Toxicol. Pharmacol.
Citation
Cheng, H., Ellis, J., Kleinow, K.M. (2015) Expression and functionality of transient receptor potential melastatin 4 (TRPM4)-like channels during development of the zebrafish. Comparative biochemistry and physiology. Toxicology & pharmacology : CBP. 178:33-44.
Abstract
Calcium signaling, from localized spikes to coordinated waves are linked to cleavage, patterning, differentiation and growth during embryonic development. The basis for control of these Ca(2+) signals is poorly defined. In this study, the expression and functionality of the Transient Receptor Potential Melastatin 4 protein (TRPM4), an ion channel that controls Ca(2+) entry into cells was examined in the zebrafish embryo and adult. Originating with the human TRPM4 gene, Ensembl orthologue, NCBI BLAST and Homologene searches identified a zebrafish TRPM4 "like" gene encoding a predicted protein of 1199 amino acids and sharing a 42 - 43% sequence identity with the mouse, rat and human. Custom designed zebrafish primers identified TRPM4 transcripts throughout the 0 - 123h period of embryonic development with greatest and lowest relative expression at 12 and 123h post fertilization, respectively. Perforated patch-clamp recordings in 27h embryonic cells revealed Ca(2+) activated currents with the characteristics of those described for mammalian TRPM4. Similarly, TRPM4-like expression and functionality was observed in brain and liver cells from adult fish. These findings suggest that a TRPM4-like channel is available for Ca(2+) regulation during early development of the zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping