PUBLICATION
SPOP mutation leads to genomic instability in prostate cancer
- Authors
- Boysen, G., Barbieri, C.E., Prandi, D., Blattner, M., Chae, S.S., Dahija, A., Nataraj, S., Huang, D., Marotz, C., Xu, L., Huang, J., Lecca, P., Chhangawala, S., Liu, D., Zhou, P., Sboner, A., de Bono, J.S., Demichelis, F., Houvras, Y., Rubin, M.A.
- ID
- ZDB-PUB-150917-5
- Date
- 2015
- Source
- eLIFE 4: (Journal)
- Registered Authors
- Houvras, Yariv
- Keywords
- Cancer Genomics, DNA repair, SPOP, cell biology, human, human biology, medicine, mouse, prostate cancer, zebrafish
- MeSH Terms
-
- Animals
- Cells, Cultured
- DNA Breaks, Double-Stranded
- DNA Damage/drug effects
- DNA Repair
- Genomic Instability*
- Humans
- Male
- Mice
- Mice, Transgenic
- Mutagens/metabolism
- Nuclear Proteins/deficiency*
- Prostatic Neoplasms/pathology*
- Repressor Proteins/deficiency*
- Zebrafish
- PubMed
- 26374986 Full text @ Elife
Citation
Boysen, G., Barbieri, C.E., Prandi, D., Blattner, M., Chae, S.S., Dahija, A., Nataraj, S., Huang, D., Marotz, C., Xu, L., Huang, J., Lecca, P., Chhangawala, S., Liu, D., Zhou, P., Sboner, A., de Bono, J.S., Demichelis, F., Houvras, Y., Rubin, M.A. (2015) SPOP mutation leads to genomic instability in prostate cancer. eLIFE. 4.
Abstract
Genomic instability is a fundamental feature of human cancer often resulting from impaired genome maintenance. In prostate cancer, structural genomic rearrangements are a common mechanism driving tumorigenesis. However, somatic alterations predisposing to chromosomal rearrangements in prostate cancer remain largely undefined. Here, we show that SPOP, the most commonly mutated gene in primary prostate cancer, modulates DNA double strand break (DSB) repair, and that SPOP mutation is associated with genomic instability. In vivo, SPOP mutation results in a transcriptional response consistent with BRCA1 inactivation resulting in impaired homology-directed repair (HDR) of DSB. Furthermore, we found that SPOP mutation sensitizes to DNA damaging therapeutic agents such as PARP inhibitors. These results implicate SPOP as a novel participant in DSB repair, suggest that SPOP mutation drives prostate tumorigenesis in part through genomic instability, and indicate that mutant SPOP may increase response to DNA damaging therapeutics.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping