PUBLICATION

Conserved Noncoding Sequences Regulate lhx5 Expression in the Zebrafish Forebrain

Authors
Sun, L., Chen, F., Peng, G.
ID
ZDB-PUB-150707-3
Date
2015
Source
PLoS One   10: e0132525 (Journal)
Registered Authors
Chen, Fengjiao, Peng, Gang, Sun, Liu
Keywords
Zebrafish, embryos, sequence alignment, fishes, vertebrates, gene regulation, amphibian genomics, gene expression
MeSH Terms
  • Animals
  • Conserved Sequence
  • Enhancer Elements, Genetic
  • Gene Expression Regulation, Developmental*
  • Gene Transfer Techniques
  • LIM-Homeodomain Proteins/genetics*
  • LIM-Homeodomain Proteins/metabolism
  • Prosencephalon/metabolism*
  • Transcription Factors/genetics*
  • Transcription Factors/metabolism
  • Zebrafish/genetics*
  • Zebrafish/metabolism
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/metabolism
(all 14)
PubMed
26147098 Full text @ PLoS One
Abstract
The LIM homeobox family protein Lhx5 plays important roles in forebrain development in the vertebrates. The lhx5 gene exhibits complex temporal and spatial expression patterns during early development but its transcriptional regulation mechanisms are not well understood. Here, we have used transgenesis in zebrafish in order to define regulatory elements that drive lhx5 expression in the forebrain. Through comparative genomic analysis we identified 10 non-coding sequences conserved in five teleost species. We next examined the enhancer activities of these conserved non-coding sequences with Tol2 transposon mediated transgenesis. We found a proximately located enhancer gave rise to robust reporter EGFP expression in the forebrain regions. In addition, we identified an enhancer located at approximately 50 kb upstream of lhx5 coding region that is responsible for reporter gene expression in the hypothalamus. We also identify an enhancer located approximately 40 kb upstream of the lhx5 coding region that is required for expression in the prethalamus (ventral thalamus). Together our results suggest discrete enhancer elements control lhx5 expression in different regions of the forebrain.
Genes / Markers
Marker Marker Type Name
dbx1aGENEdeveloping brain homeobox 1a
fam169abGENEfamily with sequence similarity 169 member Ab
lhx5GENELIM homeobox 5
mzt2bGENEmitotic spindle organizing protein 2B
nccr.lhx5.1NCCRconserved non-coding element lhx5_1
nccr.lhx5.10NCCRconserved non-coding element lhx5_10
nccr.lhx5.2NCCRconserved non-coding element lhx5_2
nccr.lhx5.3NCCRconserved non-coding element lhx5_3
nccr.lhx5.4NCCRconserved non-coding element lhx5_4
nccr.lhx5.5NCCRconserved non-coding element lhx5_5
1 - 10 of 17
Show
Figures
Figure Gallery (4 images)
Show all Figures
Expression
Phenotype
Fish Conditions Stage Phenotype Figure
WTchemical treatment: SU5402Prim-5
zf766Tgstandard conditionsPrim-5
1 - 2 of 2
Show
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
nkuasgfp1aTgTransgenic Insertion
    zf763TgTransgenic Insertion
      zf764TgTransgenic Insertion
        zf765TgTransgenic Insertion
          zf766TgTransgenic Insertion
            zf767TgTransgenic Insertion
              zf768TgTransgenic Insertion
                zf769TgTransgenic Insertion
                  zf770TgTransgenic Insertion
                    zf771TgTransgenic Insertion
                      1 - 10 of 10
                      Show
                      Human Disease / Model
                      No data available
                      Sequence Targeting Reagents
                      No data available
                      Fish
                      Fish
                      WT
                      zf763Tg
                      zf766Tg
                      zf768Tg
                      zf770Tg
                      1 - 5 of 5
                      Show
                      Antibodies
                      No data available
                      Orthology
                      No data available
                      Engineered Foreign Genes
                      Marker Marker Type Name
                      EGFPEFGEGFP
                      GAL4FFEFGGAL4FF
                      1 - 2 of 2
                      Show
                      Mapping
                      No data available
                      Your Input Welcome
                      We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate. We will review your comments promptly.
                      Citation
                      Sun, L., Chen, F., Peng, G. (2015) Conserved Noncoding Sequences Regulate lhx5 Expression in the Zebrafish Forebrain. PLoS One. 10:e0132525.