PUBLICATION
DNA damage during the G0/G1 phase triggers RNA-templated, Cockayne syndrome B-dependent homologous recombination
- Authors
- Wei, L., Nakajima, S., Böhm, S., Bernstein, K.A., Shen, Z., Tsang, M., Levine, A.S., Lan, L.
- ID
- ZDB-PUB-150624-6
- Date
- 2015
- Source
- Proceedings of the National Academy of Sciences of the United States of America 112(27): E3495-504 (Journal)
- Registered Authors
- Tsang, Michael
- Keywords
- CSB, DNA damage, RNA polymerase II, recombination, transcription
- MeSH Terms
-
- Antigens, Nuclear/genetics
- Antigens, Nuclear/metabolism
- Blotting, Western
- Cell Cycle/genetics*
- Cell Cycle Proteins/genetics
- PubMed
- 26100862 Full text @ Proc. Natl. Acad. Sci. USA
Abstract
Damage repair mechanisms at transcriptionally active sites during the G0/G1 phase are largely unknown. To elucidate these mechanisms, we introduced genome site-specific oxidative DNA damage and determined the role of transcription in repair factor assembly. We find that KU and NBS1 are recruited to damage sites independent of transcription. However, assembly of RPA1, RAD51C, RAD51, and RAD52 at such sites is strictly governed by active transcription and requires both wild-type Cockayne syndrome protein B (CSB) function and the presence of RNA in the G0/G1 phase. We show that the ATPase activity of CSB is indispensable for loading and binding of the recombination factors. CSB counters radiation-induced DNA damage in both cells and zebrafish models. Taken together, our results have uncovered a novel, RNA-based recombination mechanism by which CSB protects genome stability from strand breaks at transcriptionally active sites and may provide insight into the clinical manifestations of Cockayne syndrome.
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Sequence Targeting Reagents
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