PUBLICATION

First Demonstration of Antigen Induced Cytokine Expression by CD4-1+ Lymphocytes in a Poikilotherm: Studies in Zebrafish (Danio rerio)

Authors
Yoon, S., Mitra, S., Wyse, C., Alnabulsi, A., Zou, J., Weerdenburg, E.M., van der Sar, A.M., Wang, D., Secombes, C.J., Bird, S.
ID
ZDB-PUB-150618-2
Date
2015
Source
PLoS One   10: e0126378 (Journal)
Registered Authors
van der Sar, Astrid M., Zou, Jun
Keywords
Zebrafish, Cytokines, Lymphocytes, T cells, Kidneys, White blood cells, Gene expression, Polymerase chain reaction
MeSH Terms
  • Adaptive Immunity
  • Amino Acid Sequence
  • Animals
  • Antibodies/chemistry
  • CD4-Positive T-Lymphocytes/classification
  • CD4-Positive T-Lymphocytes/immunology*
  • CD4-Positive T-Lymphocytes/microbiology
  • CD4-Positive T-Lymphocytes/pathology
  • Cytokines/biosynthesis*
  • Cytokines/immunology
  • Genetic Loci/immunology
  • Histocompatibility Antigens Class II/genetics
  • Histocompatibility Antigens Class II/immunology*
  • Humans
  • Models, Molecular
  • Molecular Sequence Data
  • Mycobacterium Infections, Nontuberculous/genetics
  • Mycobacterium Infections, Nontuberculous/immunology*
  • Mycobacterium Infections, Nontuberculous/microbiology
  • Mycobacterium Infections, Nontuberculous/veterinary*
  • Mycobacterium marinum/immunology
  • Phylogeny
  • RNA, Messenger/genetics
  • RNA, Messenger/immunology*
  • Sequence Alignment
  • Th1-Th2 Balance
  • ZAP-70 Protein-Tyrosine Kinase/genetics
  • ZAP-70 Protein-Tyrosine Kinase/immunology
  • Zebrafish/classification
  • Zebrafish/genetics
  • Zebrafish/immunology
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/immunology
  • gamma-Globulins/administration & dosage
(all 34)
PubMed
26083432 Full text @ PLoS One
Abstract
Adaptive immunity in homeotherms depends greatly on CD4+ Th cells which release cytokines in response to specific antigen stimulation. Whilst bony fish and poikilothermic tetrapods possess cells that express TcR and CD4-related genes (that exist in two forms in teleost fish; termed CD4-1 and CD4-2), to date there is no unequivocal demonstration that cells equivalent to Th exist. Thus, in this study we determined whether CD4-1+ lymphocytes can express cytokines typical of Th cells following antigen specific stimulation, using the zebrafish (Danio rerio). Initially, we analyzed the CD4 locus in zebrafish and found three CD4 homologues, a CD4-1 molecule and two CD4-2 molecules. The zfCD4-1 and zfCD4-2 transcripts were detected in immune organs and were most highly expressed in lymphocytes. A polyclonal antibody to zfCD4-1 was developed and used with an antibody to ZAP70 and revealed double positive cells by immunohistochemistry, and in the Mycobacterium marinum disease model CD4-1+ cells were apparent surrounding the granulomas typical of the infection. Next a prime-boost experiment, using human gamma globulin as antigen, was performed and revealed for the first time in fish that zfCD4-1+ lymphocytes increase the expression of cytokines and master transcription factors relevant to Th1/Th2-type responses as a consequence of boosting with specific antigen.
Errata / Notes
Correction.
Genes / Markers
Figures
Figure Gallery (3 images)
Show all Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Your Input Welcome
We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate. We will review your comments promptly.
Citation
Yoon, S., Mitra, S., Wyse, C., Alnabulsi, A., Zou, J., Weerdenburg, E.M., van der Sar, A.M., Wang, D., Secombes, C.J., Bird, S. (2015) First Demonstration of Antigen Induced Cytokine Expression by CD4-1+ Lymphocytes in a Poikilotherm: Studies in Zebrafish (Danio rerio). PLoS One. 10:e0126378.