PUBLICATION

Radial glial cell-specific ablation in the adult zebrafish brain

Authors
Shimizu, Y., Ito, Y., Tanaka, H., Ohshima, T.
ID
ZDB-PUB-150606-4
Date
2015
Source
Genesis (New York, N.Y. : 2000)   53(7): 431-9 (Journal)
Registered Authors
Ohshima, Toshio, Shimizu, Yuki, Tanaka, Hideomi
Keywords
Gal4-UAS, adult neurogenesis, cell ablation, transgenic, zebrafish
MeSH Terms
  • Ablation Techniques/methods
  • Animals
  • Animals, Genetically Modified
  • Brain/cytology*
  • Brain/metabolism
  • Dentate Gyrus/cytology
  • Dentate Gyrus/metabolism
  • Ependymoglial Cells/cytology*
  • Ependymoglial Cells/metabolism
  • Glial Fibrillary Acidic Protein/biosynthesis
  • Glial Fibrillary Acidic Protein/genetics
  • Neural Stem Cells/cytology
  • Neural Stem Cells/metabolism
  • Neurogenesis/physiology
  • Zebrafish
PubMed
26045148 Full text @ Genesis
Citation
Shimizu, Y., Ito, Y., Tanaka, H., Ohshima, T. (2015) Radial glial cell-specific ablation in the adult zebrafish brain. Genesis (New York, N.Y. : 2000). 53(7):431-9.
Abstract
The zebrafish brain can continue to produce new neurons in widespread neurogenic brain regions throughout life. In contrast, neurogenesis in the adult mammalian brain is restricted to the subventricular zone (SVZ) and dentate gyrus (DG). In neurogenic regions in the adult brain, radial glial cells (RGCs) are considered to function as neural stem cells (NSCs). We generated a Tg(gfap:Gal4FF) transgenic zebrafish line, which enabled us to express specific genes in RGCs. To study the function of RGCs in neurogenesis in the adult zebrafish brain, we also generated a Tg(gfap:Gal4FF;UAS:nfsB-mcherry) transgenic zebrafish line, which allowed us to induce cell death exclusively within RGCs upon addition of metronidazole (Mtz) to the media. RGCs expressing nitroreductase were specifically ablated by the Mtz treatment, decreasing the number of proliferative RGCs. Using the Tg(gfap:Gal4FF;UAS:nfsB-mcherry) transgenic zebrafish line, we found that RGCs were specifically ablated in the adult zebrafish telencephalon. The Tg(gfap:Gal4FF) line could be useful to study the function of RGCs. This article is protected by copyright. All rights reserved.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
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