PUBLICATION

Control of brain patterning by Engrailed paracrine transfer: a new function of the Pbx interaction domain

Authors
Rampon, C., Gauron, C., Lin, T., Meda, F., Dupont, E., Cosson, A., Ipendey, E., Frerot, A., Aujard, I., Le Saux, T., Bensimon, D., Jullien, L., Volovitch, M., Vriz, S., Joliot, A.
ID
ZDB-PUB-150501-2
Date
2015
Source
Development (Cambridge, England)   142(10): 1840-9 (Journal)
Registered Authors
Bensimon, David, Gauron, Carole, Rampon, Christine, Vriz, Sophie
Keywords
none
MeSH Terms
  • Animals
  • Brain/embryology*
  • Brain/metabolism*
  • Homeodomain Proteins/genetics
  • Homeodomain Proteins/metabolism
  • Nerve Tissue Proteins/genetics
  • Nerve Tissue Proteins/metabolism
  • Zebrafish/embryology*
  • Zebrafish/metabolism*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
(all 11)
PubMed
25926358 Full text @ Development
Abstract
Homeoproteins of the Engrailed family are involved in the patterning of mesencephalic boundaries through a mechanism classically ascribed to their transcriptional functions. In light of recent reports on the paracrine activity of homeoproteins, including Engrailed, we asked whether Engrailed intercellular transfer was also involved in brain patterning and boundary formation. Using time-controlled activation of Engrailed combined with tools that block its transfer, we show that the positioning of the diencephalic-mesencephalic boundary (DMB) requires Engrailed paracrine activity. Both zebrafish Eng2a and Eng2b are competent for intercellular transfer in vivo, but only extracellular endogenous Eng2b, and not Eng2a, participates in DMB positioning. In addition, disruption of the Pbx-interacting motif in Engrailed, known to strongly reduce the gain-of-function phenotype, also downregulates Engrailed transfer, thus revealing an unsuspected participation of the Pbx interaction domain in this pathway.
Genes / Markers
Figures
Figure Gallery (4 images)
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Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
pku2EtTransgenic Insertion
    vu12TgTransgenic Insertion
      zf371EtTransgenic Insertion
        1 - 3 of 3
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        Human Disease / Model
        No data available
        Sequence Targeting Reagents
        No data available
        Fish
        Fish
        pku2Et
        vu12Tg
        zf371Et
        1 - 3 of 3
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        Antibodies
        Orthology
        Engineered Foreign Genes
        Marker Marker Type Name
        EGFPEFGEGFP
        GFPEFGGFP
        1 - 2 of 2
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        Mapping
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        Citation
        Rampon, C., Gauron, C., Lin, T., Meda, F., Dupont, E., Cosson, A., Ipendey, E., Frerot, A., Aujard, I., Le Saux, T., Bensimon, D., Jullien, L., Volovitch, M., Vriz, S., Joliot, A. (2015) Control of brain patterning by Engrailed paracrine transfer: a new function of the Pbx interaction domain. Development (Cambridge, England). 142(10):1840-9.