PUBLICATION
Early steps in primary cilium assembly require EHD1/EHD3-dependent ciliary vesicle formation
- Authors
- Lu, Q., Insinna, C., Ott, C., Stauffer, J., Pintado, P.A., Rahajeng, J., Baxa, U., Walia, V., Cuenca, A., Hwang, Y.S., Daar, I.O., Lopes, S., Lippincott-Schwartz, J., Jackson, P.K., Caplan, S., Westlake, C.J.
- ID
- ZDB-PUB-150429-18
- Date
- 2015
- Source
- Nature cell biology 17: 228-40 (Journal)
- Registered Authors
- Lopes, Susana
- Keywords
- none
- MeSH Terms
-
- Animals
- Axoneme/metabolism
- Axoneme/ultrastructure
- Carrier Proteins/antagonists & inhibitors
- Carrier Proteins/genetics*
- Carrier Proteins/metabolism
- Cell Line
- Centrioles/metabolism
- Centrioles/ultrastructure
- Cilia/metabolism*
- Cilia/ultrastructure
- Embryo, Nonmammalian
- Epithelial Cells/metabolism*
- Epithelial Cells/ultrastructure
- Gene Expression Regulation, Developmental*
- Humans
- Kidney Tubules, Collecting/metabolism
- Kidney Tubules, Collecting/ultrastructure
- Mice
- Morphogenesis/genetics
- Qb-SNARE Proteins/genetics
- Qb-SNARE Proteins/metabolism
- Qc-SNARE Proteins/genetics
- Qc-SNARE Proteins/metabolism
- RNA, Small Interfering/genetics
- RNA, Small Interfering/metabolism
- Retinal Pigment Epithelium/metabolism
- Retinal Pigment Epithelium/ultrastructure
- Signal Transduction
- Transport Vesicles/metabolism*
- Transport Vesicles/ultrastructure
- Vesicular Transport Proteins/antagonists & inhibitors
- Vesicular Transport Proteins/genetics*
- Vesicular Transport Proteins/metabolism
- Zebrafish
- rab GTP-Binding Proteins/genetics
- rab GTP-Binding Proteins/metabolism
- PubMed
- 25686250 Full text @ Nat. Cell Biol.
Citation
Lu, Q., Insinna, C., Ott, C., Stauffer, J., Pintado, P.A., Rahajeng, J., Baxa, U., Walia, V., Cuenca, A., Hwang, Y.S., Daar, I.O., Lopes, S., Lippincott-Schwartz, J., Jackson, P.K., Caplan, S., Westlake, C.J. (2015) Early steps in primary cilium assembly require EHD1/EHD3-dependent ciliary vesicle formation. Nature cell biology. 17:228-40.
Abstract
Membrane association with mother centriole (M-centriole) distal appendages is critical for ciliogenesis initiation. How the Rab GTPase Rab11-Rab8 cascade functions in early ciliary membrane assembly is unknown. Here, we show that the membrane shaping proteins EHD1 and EHD3, in association with the Rab11-Rab8 cascade, function in early ciliogenesis. EHD1 and EHD3 localize to preciliary membranes and the ciliary pocket. EHD-dependent membrane tubulation is essential for ciliary vesicle formation from smaller distal appendage vesicles (DAVs). Importantly, this step functions in M-centriole to basal body transformation and recruitment of transition zone proteins and IFT20. SNAP29, a SNARE membrane fusion regulator and EHD1-binding protein, is also required for DAV-mediated ciliary vesicle assembly. Interestingly, only after ciliary vesicle assembly is Rab8 activated for ciliary growth. Our studies uncover molecular mechanisms informing a previously uncharacterized ciliogenesis step, whereby EHD1 and EHD3 reorganize the M-centriole and associated DAVs before coordinated ciliary membrane and axoneme growth.
Errata / Notes
This article is corrected by ZDB-PUB-220906-14.
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