PUBLICATION
Effects of lorazepam and WAY-200070 in larval zebrafish light/dark choice test
- Authors
- Chen, F., Chen, S., Liu, S., Zhang, C., Peng, G.
- ID
- ZDB-PUB-150407-7
- Date
- 2015
- Source
- Neuropharmacology 95: 226-33 (Journal)
- Registered Authors
- Chen, Fengjiao, Chen, Sijie, Peng, Gang, Zhang, Cuizhen
- Keywords
- anxiety, benzodiazepines, estrogen receptor beta, habenula, zebrafish
- MeSH Terms
-
- Animals
- Anti-Anxiety Agents/pharmacology*
- Anxiety/drug therapy*
- Anxiety/metabolism
- Boron Compounds/pharmacology
- Choice Behavior/drug effects
- Choice Behavior/physiology
- Diazepam/pharmacology
- Estradiol/analogs & derivatives
- Estradiol/pharmacology
- Estrogen Receptor beta/metabolism
- Habenula/drug effects
- Habenula/metabolism
- Hypothalamus/drug effects
- Hypothalamus/metabolism
- Larva
- Light
- Lorazepam/pharmacology*
- Models, Animal
- Neuropsychological Tests
- Oxazoles/pharmacology*
- Phenols/pharmacology*
- Phenylalanine/analogs & derivatives
- Phenylalanine/pharmacology
- Proto-Oncogene Proteins c-fos/metabolism
- Pyrazoles/pharmacology
- Pyrimidines/pharmacology
- Visual Perception/drug effects
- Visual Perception/physiology
- Zebrafish
- Zebrafish Proteins/metabolism
- PubMed
- 25842247 Full text @ Neuropharmacology
Citation
Chen, F., Chen, S., Liu, S., Zhang, C., Peng, G. (2015) Effects of lorazepam and WAY-200070 in larval zebrafish light/dark choice test. Neuropharmacology. 95:226-33.
Abstract
Zebrafish larvae spend more time in brightly illuminated area when placed in a light/dark testing environment. Here we report that the anxiolytic drugs lorazepam and diazepam increased the time larval fish spent in the dark compartment in the light/dark test. Lorazepam did not affect the visual induced optokinetic response of larval fish. Gene expression levels of c-fos and crh were significantly increased in the hypothalamus of fish larvae underwent light/dark choice behavior, whilst lorazepam treatment alleviated the increased c-fos and crh expressions. Furthermore, we found estrogen receptor β gene expression level was increased in fish larvae underwent light/dark choice. We next examined effects of estrogen receptor modulators (estradiol, BPA, PHTPP, and WAY-200070) in the light/dark test. We identified WAY-200070, a highly selective ERβ agonist significantly altered the light/dark choice behavior of zebrafish larvae. Further investigation showed WAY-200070 treatment caused a reduction of crh expression level in the hypothalamus, suggesting activation of ERβ signaling attenuate the stress response. Interestingly, WAY-200070 treatment caused marked increase of c-fos expression in the habenula of fish larvae underwent behavior test. These results suggest WAY-200070 activation of ERβ mediated signaling may regulate anxiety related behavior in zebrafish through modulation of neuronal activity in habenula.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping