PUBLICATION
The secreted factor Ag1 missing in higher vertebrates regulates fins regeneration in Danio rerio
- Authors
- Ivanova, A.S., Shandarin, I.N., Ermakova, G.V., Minin, A.A., Tereshina, M.B., Zaraisky, A.G.
- ID
- ZDB-PUB-150130-3
- Date
- 2015
- Source
- Scientific Reports 5: 8123 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Animal Fins/physiology*
- Animals
- Apoptosis/drug effects
- DNA, Complementary/genetics
- Embryo, Nonmammalian/metabolism
- Embryonic Development/genetics
- Gene Expression Regulation, Developmental
- In Situ Hybridization
- In Situ Nick-End Labeling
- Injections
- Morpholinos/administration & dosage
- Morpholinos/pharmacology
- Polymerase Chain Reaction
- Protein Biosynthesis
- Protein Disulfide-Isomerases/genetics
- Protein Disulfide-Isomerases/metabolism*
- RNA, Messenger/genetics
- RNA, Messenger/metabolism
- Regeneration*/genetics
- Time Factors
- Zebrafish/embryology
- Zebrafish/genetics
- Zebrafish/metabolism*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 25630240 Full text @ Sci. Rep.
Citation
Ivanova, A.S., Shandarin, I.N., Ermakova, G.V., Minin, A.A., Tereshina, M.B., Zaraisky, A.G. (2015) The secreted factor Ag1 missing in higher vertebrates regulates fins regeneration in Danio rerio. Scientific Reports. 5:8123.
Abstract
Agr family includes three groups of genes, Ag1, Agr2 and Agr3, which encode the thioredoxin domain-containing secreted proteins and have been shown recently to participate in regeneration of the amputated body appendages in amphibians. By contrast, higher vertebrates have only Agr2 and Agr3, but lack Ag1, and have low ability to regenerate the body appendages. Thus, one may hypothesize that loss of Ag1 in evolution could be an important event that led to a decline of the regenerative capacity in higher vertebrates. To test this, we have studied now the expression and role of Ag1 in the regeneration of fins of a representative of another large group of lower vertebrates, the fish Danio rerio. As a result, we have demonstrated that amputation of the Danio fins, like amputation of the body appendages in amphibians, elicits an increase of Ag1 expression in cells of the stump. Furthermore, down-regulation of DAg1 by injections of Vivo-morpholino antisense oligonucleotides resulted in a retardation of the fin regeneration. These data are in a good agreement with the assumption that the loss of Ag1 in higher vertebrates ancestors could lead to the reduction of the regenerative capacity in their modern descendants.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping