PUBLICATION
Effect of titanium dioxide nanoparticles on zebrafish embryos and developing retina
- Authors
- Wang, Y.J., He, Z.Z., Fang, Y.W., Xu, Y., Chen, Y.N., Wang, G.Q., Yang, Y.Q., Yang, Z., Li, Y.H.
- ID
- ZDB-PUB-141230-18
- Date
- 2014
- Source
- International journal of ophthalmology 7: 917-23 (Journal)
- Registered Authors
- Li, Yuhao
- Keywords
- microglia, retina, titanium dioxide nanoparticles, zebrafish
- MeSH Terms
- none
- PubMed
- 25540739 Full text @ Int J Ophthalmol
Citation
Wang, Y.J., He, Z.Z., Fang, Y.W., Xu, Y., Chen, Y.N., Wang, G.Q., Yang, Y.Q., Yang, Z., Li, Y.H. (2014) Effect of titanium dioxide nanoparticles on zebrafish embryos and developing retina. International journal of ophthalmology. 7:917-23.
Abstract
Aim To investigate the impact of titanium dioxide nanoparticles (TiO2 NPs) on embryonic development and retinal neurogenesis.
Methods The agglomeration and sedimentation of TiO2 NPs solutions at different dilutions were observed, and the ultraviolet-visible spectra of their supernatants were measured. Zebrafish embryos were experimentally exposed to TiO2 NPs until 72h postfertilization (hpf). The retinal neurogenesis and distribution of the microglia were analyzed by immunohistochemistry and whole mount in situ hybridization.
Results The 1 mg/L was determined to be an appropriate exposure dose. Embryos exposed to TiO2 NPs had a normal phenotype. The neurogenesis was initiated on time, and ganglion cells, cones and rods were well differentiated at 72 hpf. The expression of fms mRNA and the 4C4 antibody, which were specific to microglia in the central nervous system (CNS), closely resembled their endogenous profile.
Conclusion These data demonstrate that short-term exposure to TiO2 NPs at a low dose does not lead to delayed embryonic development or retinal neurotoxicity.
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