PUBLICATION
Interplay of Cell Shape and Division Orientation Promotes Robust Morphogenesis of Developing Epithelia
- Authors
- Xiong, F., Ma, W., Hiscock, T.W., Mosaliganti, K.R., Tentner, A.R., Brakke, K.A., Rannou, N., Gelas, A., Souhait, L., Swinburne, I.A., Obholzer, N.D., Megason, S.G.
- ID
- ZDB-PUB-141011-2
- Date
- 2014
- Source
- Cell 159: 415-427 (Journal)
- Registered Authors
- Gelas, Arnaud, Megason, Sean, Mosaliganti, Kishore, Obholzer, Nikolaus, Souhait, Lydie, Swinburne, Ian, Tentner, Andrea, Xiong, Fengzhu
- Keywords
- none
- MeSH Terms
-
- Animals
- Biomechanical Phenomena
- Cell Count
- Cell Division
- Cell Shape
- Embryo, Nonmammalian/cytology
- Epithelial Cells/cytology*
- Models, Biological*
- Morphogenesis*
- Zebrafish/embryology*
- PubMed
- 25303534 Full text @ Cell
Citation
Xiong, F., Ma, W., Hiscock, T.W., Mosaliganti, K.R., Tentner, A.R., Brakke, K.A., Rannou, N., Gelas, A., Souhait, L., Swinburne, I.A., Obholzer, N.D., Megason, S.G. (2014) Interplay of Cell Shape and Division Orientation Promotes Robust Morphogenesis of Developing Epithelia. Cell. 159:415-427.
Abstract
Epithelial cells acquire functionally important shapes (e.g., squamous, cuboidal, columnar) during development. Here, we combine theory, quantitative imaging, and perturbations to analyze how tissue geometry, cell divisions, and mechanics interact to shape the presumptive enveloping layer (pre-EVL) on the zebrafish embryonic surface. We find that, under geometrical constraints, pre-EVL flattening is regulated by surface cell number changes following differentially oriented cell divisions. The division pattern is, in turn, determined by the cell shape distribution, which forms under geometrical constraints by cell-cell mechanical coupling. An integrated mathematical model of this shape-division feedback loop recapitulates empirical observations. Surprisingly, the model predicts that cell shape is robust to changes of tissue surface area, cell volume, and cell number, which we confirm in vivo. Further simulations and perturbations suggest the parameter linking cell shape and division orientation contributes to epithelial diversity. Together, our work identifies an evolvable design logic that enables robust cell-level regulation of tissue-level development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping