PUBLICATION
Differential expression of synapsin genes during early zebrafish development
- Authors
- Garbarino, G., Costa, S., Pestarino, M., Candiani, S.
- ID
- ZDB-PUB-140923-15
- Date
- 2014
- Source
- Neuroscience 280: 351-67 (Journal)
- Registered Authors
- Keywords
- Danio rerio, In situ hybridization, Nervous system, Syn locus evolution, Synapsin
- MeSH Terms
-
- Animals
- Biological Evolution
- Gene Expression Regulation, Developmental
- In Situ Hybridization
- Molecular Sequence Data
- PubMed
- 25239370 Full text @ Neuroscience
Abstract
Synapsins are a family of synaptic vesicle (SV)-associated phosphoproteins that have been identified in several vertebrates and invertebrates. We report here the cloning and expression of synapsin family genes in the zebrafish Danio rerio. We identified the complete coding sequence of synapsin 3, which is not present in the currently available genome, and characterized and annotated the synapsin gene family in the zebrafish Danio rerio. By means of whole-mount in situ hybridization, we showed the spatiotemporal expression of synapsin genes at three different time points during early embryonic development: 20 to 24 hours postfertilization (hpf), 30 to 33 hpf, and 3 days postfertilization (dpf). As very few data are available describing the expression of synapsin family genes during CNS development in vertebrate models, our results may help to achieve a better understanding of the complex functions of these molecules. Finally, new interesting evidence from our temporal gene expression studies suggests that synapsins have also maternal functions.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping