PUBLICATION
Role of intestinal inflammation in predisposition of Edwardsiella tarda infection in zebrafish (Danio rerio)
- Authors
- Liu, X., Chang, X., Wu, H., Xiao, J., Gao, Y., Zhang, Y.
- ID
- ZDB-PUB-140917-3
- Date
- 2014
- Source
- Fish & shellfish immunology 41(2): 271-8 (Journal)
- Registered Authors
- Keywords
- Edwardsiella tarda, antimicrobial response, infection, intestinal inflammation, zebrafish
- MeSH Terms
-
- Animals
- China
- DNA Primers/genetics
- Disease Susceptibility/microbiology
- Disease Susceptibility/physiopathology*
- Edwardsiella tarda/immunology*
- Enterobacteriaceae Infections/immunology
- Enterobacteriaceae Infections/physiopathology
- Enterobacteriaceae Infections/veterinary*
- Fish Diseases/immunology
- Fish Diseases/microbiology*
- Fish Diseases/physiopathology
- Gene Expression Regulation/immunology
- Inflammation/physiopathology*
- Intestines/physiopathology
- Real-Time Polymerase Chain Reaction
- Zebrafish*
- PubMed
- 25224880 Full text @ Fish Shellfish Immunol.
Citation
Liu, X., Chang, X., Wu, H., Xiao, J., Gao, Y., Zhang, Y. (2014) Role of intestinal inflammation in predisposition of Edwardsiella tarda infection in zebrafish (Danio rerio). Fish & shellfish immunology. 41(2):271-8.
Abstract
Edwardsiella tarda, an enteric opportunistic pathogen, is associated with acute to chronic edwardsiellosis in cultured fish, resulting in heavy losses in aquaculture. To date, the pathogenesis of E. tarda has been extensively studied and a great deal of vaccine candidates have been attempted. However, the research on the predisposition of E. tarda infection is poorly reported. In this study, the effects of intestinal inflammation on E. tarda infection were investigated using a zebrafish model that influenced by perturbation of intestinal microbiota. Featured symptoms of edwardsiellosis were observed in intestinal inflammatory zebrafish compared with healthy fish. Higher bacterial numbers were detected in both mucosal tissues (intestine, skin and gills) and lymphoid tissues (liver, spleen and kidney) of inflammatory zebrafish while the bacterial loads in healthy zebrafish appeared to be relatively lower by 10-100 folds. Moreover, significant up-regulation of IL-1β, TNF-α and iNOS was noticed in multiple tissues of zebrafish with intestinal inflammation between 6 and 72 h post infection. However, only moderate elevation was observed in the gills and liver of healthy fish. Furthermore, the expression of genes involved in neutrophil recruitment (mpx, IL-8 and LECT2) and antimicrobial response (β-defensin and hepcidin) showed notable up-regulation in the intestine of inflammatory zebrafish. These results demonstrate that fish with intestinal inflammation is more susceptible to E. tarda and the antimicrobial response during E. tarda infection might inhibit the growth of intestinal microbiota that E. tarda could resist. Our results suggest that maintaining good management to avoid intestinal inflammation is a feasible prevention measure against edwardsiellosis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping