PUBLICATION
Blood Flow and Bmp Signaling Control Endocardial Chamber Morphogenesis
- Authors
- Dietrich, A.C., Lombardo, V.A., Abdelilah-Seyfried, S.
- ID
- ZDB-PUB-140828-7
- Date
- 2014
- Source
- Developmental Cell 30: 367-377 (Journal)
- Registered Authors
- Abdelilah-Seyfried, Salim
- Keywords
- none
- MeSH Terms
-
- Animals
- Bone Morphogenetic Proteins/metabolism*
- Cell Movement
- Cell Proliferation
- Endocardium/cytology
- Endocardium/embryology*
- Endocardium/metabolism
- Endothelial Cells/metabolism
- Endothelial Cells/physiology
- Hemodynamics*
- Kruppel-Like Transcription Factors/genetics
- Kruppel-Like Transcription Factors/metabolism*
- Morphogenesis*
- Signal Transduction
- Vascular Endothelial Growth Factor A/metabolism
- Zebrafish
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 25158852 Full text @ Dev. Cell
Citation
Dietrich, A.C., Lombardo, V.A., Abdelilah-Seyfried, S. (2014) Blood Flow and Bmp Signaling Control Endocardial Chamber Morphogenesis. Developmental Cell. 30:367-377.
Abstract
During heart development, the onset of heartbeat and blood flow coincides with a ballooning of the cardiac chambers. Here, we have used the zebrafish as a vertebrate model to characterize chamber ballooning morphogenesis of the endocardium, a specialized population of endothelial cells that line the interior of the heart. By combining functional manipulations, fate mapping studies, and high-resolution imaging, we show that endocardial growth occurs without an influx of external cells. Instead, endocardial cell proliferation is regulated, both by blood flow and by Bmp signaling, in a manner independent of vascular endothelial growth factor (VEGF) signaling. Similar to myocardial cells, endocardial cells obtain distinct chamber-specific and inner- versus outer-curvature-specific surface area sizes. We find that the hemodynamic-sensitive transcription factor Klf2a is involved in regulating endocardial cell morphology. These findings establish the endocardium as the flow-sensitive tissue in the heart with a key role in adapting chamber growth in response to the mechanical stimulus of blood flow.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping