PUBLICATION
Characterization of Bbx, a member of a novel subfamily of the HMG-box superfamily together with Cic
- Authors
- Chen, T., Zhou, L., Yuan, Y., Fang, Y., Guo, Y., Huang, H., Zhou, Q., Lv, X.
- ID
- ZDB-PUB-140801-10
- Date
- 2014
- Source
- Development genes and evolution 224(4-6): 261-8 (Journal)
- Registered Authors
- Huang, Huizhe, Zhou, Li
- Keywords
- none
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Gene Expression
- High Mobility Group Proteins/chemistry
- High Mobility Group Proteins/genetics*
- High Mobility Group Proteins/metabolism
- Mice
- Molecular Sequence Data
- Phylogeny
- Sequence Alignment
- Transcription Factors/chemistry
- Transcription Factors/genetics*
- Transcription Factors/metabolism
- Zebrafish/embryology
- Zebrafish/genetics*
- PubMed
- 25079045 Full text @ Dev. Genes Evol.
Citation
Chen, T., Zhou, L., Yuan, Y., Fang, Y., Guo, Y., Huang, H., Zhou, Q., Lv, X. (2014) Characterization of Bbx, a member of a novel subfamily of the HMG-box superfamily together with Cic. Development genes and evolution. 224(4-6):261-8.
Abstract
High mobility group (HMG)-box proteins, a large and functionally diverse superfamily of architectural protein, are involved in the regulation of DNA-dependent processes such as transcription, replication and DNA repair via the HMG-box domain. Bobby sox homolog (BBX), a newly identified HMG-Box protein, may function as a sequence-specific transcription factor. However, its expression pattern and biological functions are largely unknown. In this work, phylogenetic analysis showed that BBX is highly conserved and belongs to a novel subfamily of HMG-Box superfamily together with CIC (capicua homolog). Real time RT-PCR and whole-mount in situ hybridization in zebrafish embryo revealed that bbx, cica, and cicb were maternally highly expressed from 4 cell to 1K cell stage, and the zygote expression was primarily distributed in the central nervous system (CNS) from 24 to 60 h post-fertilization (hpf). Immunohistochemistry analysis in mouse brain revealed that BBX was weakly expressed in the cerebellum and highly expressed in the cortex and hippocampus. These findings indicate that as a novel HMG-box protein, BBX maybe associated with CNS development and provides useful clues to further study of its biological functions.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping