PUBLICATION

The role of inab in axon morphology of an identified zebrafish motoneuron

Authors
Van Ryswyk, L., Simonson, L., and Eisen, J.S.
ID
ZDB-PUB-140509-10
Date
2014
Source
PLoS One   9(2): e88631 (Journal)
Registered Authors
Eisen, Judith S.
Keywords
none
MeSH Terms
  • Animals
  • Axons/physiology*
  • Cell Differentiation
  • Gene Expression Profiling
  • Gene Expression Regulation, Developmental
  • Green Fluorescent Proteins/metabolism
  • In Situ Hybridization, Fluorescence
  • Interneurons/cytology
  • Motor Neurons/cytology*
  • Neurofilament Proteins/physiology*
  • Oligonucleotide Array Sequence Analysis
  • Spinal Cord/embryology*
  • Spinal Cord/pathology
  • Transcription, Genetic
  • Zebrafish
  • Zebrafish Proteins/physiology*
(all 16)
PubMed
24533123 Full text @ PLoS One
Abstract

The ability of an animal to move and to interact with its environment requires that motoneurons correctly innervate specific muscles. Although many genes that regulate motoneuron development have been identified, our understanding of motor axon branching remains incomplete. We used transcriptional expression profiling to identify potential candidate genes involved in development of zebrafish identified motoneurons. Here we focus on inab, an intermediate filament encoding gene dynamically expressed in a subset of motoneurons as well as in an identified interneuron. We show that inab is necessary for proper axon morphology of a specific motoneuron subtype.

Genes / Markers
Marker Marker Type Name
ccdc85alGENEcoiled-coil domain containing 85A, like
gad1bGENEglutamate decarboxylase 1b
gad2GENEglutamate decarboxylase 2
inaaGENEinternexin neuronal intermediate filament protein, alpha a
inabGENEinternexin neuronal intermediate filament protein, alpha b
isl1aGENEISL LIM homeobox 1a
isl2aGENEISL LIM homeobox 2a
mnx1GENEmotor neuron and pancreas homeobox 1
mnx2aGENEmotor neuron and pancreas homeobox 2a
mnx2bGENEmotor neuron and pancreas homeobox 2b
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Figures
Figure Gallery (6 images)
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Expression
Phenotype
Fish Conditions Stage Phenotype Figure
b1222Tg; b1224Tg + MO1-inab + MO2-inabstandard conditionsPrim-25
1 - 1 of 1
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Mutations / Transgenics
Allele Construct Type Affected Genomic Region
b641
    		Point Mutation
    b1222TgTransgenic Insertion
      b1224TgTransgenic Insertion
        js12TgTransgenic Insertion
          ml2TgTransgenic Insertion
            nns5TgTransgenic Insertion
              1 - 6 of 6
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              Human Disease / Model
              No data available
              Sequence Targeting Reagents
              Target Reagent Reagent Type
              inabMO1-inabMRPHLNO
              inabMO2-inabMRPHLNO
              inabMO3-inabMRPHLNO
              mnx1MO5-mnx1MRPHLNO
              mnx2aMO1-mnx2aMRPHLNO
              mnx2bMO1-mnx2bMRPHLNO
              1 - 6 of 6
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              Fish
              Fish
              b1222Tg
              b1222Tg; b1224Tg + MO1-inab + MO2-inab
              b1224Tg
              js12Tg
              js12Tg + MO1-mnx2a + MO1-mnx2b + MO5-mnx1
              ml2Tg
              ml2Tg + MO1-inab + MO2-inab
              nns5Tg
              smob641/b641
              WT
              1 - 10 of 10
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              Antibodies
              Orthology
              No data available
              Engineered Foreign Genes
              Marker Marker Type Name
              EGFPEFGEGFP
              GAL4EFGGAL4
              GFPEFGGFP
              TomatoEFGTomato
              1 - 4 of 4
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              Mapping
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              Citation
              Van Ryswyk, L., Simonson, L., and Eisen, J.S. (2014) The role of inab in axon morphology of an identified zebrafish motoneuron. PLoS One. 9(2):e88631.