PUBLICATION
Quantitative measurement of blood velocity in zebrafish with optical vector field tomography
- Authors
- Fieramonti, L., Foglia, E.A., Malavasi, S., D'Andrea, C., Valentini, G., Cotelli, F., and Bassi, A.
- ID
- ZDB-PUB-140213-10
- Date
- 2015
- Source
- Journal of biophotonics 8(1-2): 52-9 (Journal)
- Registered Authors
- Cotelli, Franco
- Keywords
- none
- MeSH Terms
-
- Animals
- Blood Circulation*
- Imaging, Three-Dimensional
- Tomography, Optical/methods*
- Zebrafish/physiology*
- PubMed
- 24339189 Full text @ J. Biophotonics
Citation
Fieramonti, L., Foglia, E.A., Malavasi, S., D'Andrea, C., Valentini, G., Cotelli, F., and Bassi, A. (2015) Quantitative measurement of blood velocity in zebrafish with optical vector field tomography. Journal of biophotonics. 8(1-2):52-9.
Abstract
Microscopy techniques can readily visualize the finest details of embryo vasculature, but still lack to provide a complete three-dimensional representation of blood flow parameters. We present an in-vivo 3D imaging technique, able to reconstruct the blood cell velocity vector over a large volume of zebrafish embryos. This low cost and relatively simple technique is exploited to quantitatively assess blood velocity in the zebrafish tail at different stages of development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping