A Dinuclear Ruthenium(II) Complex as a One- and Two-Photon Luminescent Probe for Biological Cu2+ Detection
- Authors
- Zhang, P., Pei, L., Chen, Y., Xu, W., Lin, Q., Wang, J., Wu, J., Shen, Y., Ji, L., and Chao, H.
- ID
- ZDB-PUB-131121-31
- Date
- 2013
- Source
- Chemistry (Weinheim an der Bergstrasse, Germany) 19(46): 15494-503 (Journal)
- Registered Authors
- Chen, Yu
- Keywords
- copper, living cells, luminescent probe, ruthenium, two-photon excitation, zebrafish
- MeSH Terms
-
- 2,2'-Dipyridyl/chemistry*
- Animals
- Cell Line
- Coordination Complexes/chemical synthesis*
- Coordination Complexes/chemistry
- Copper/analysis*
- Copper/chemistry*
- Diamines/chemical synthesis*
- Diamines/chemistry
- Fluorescent Dyes/chemistry*
- Humans
- Luminescence
- Molecular Structure
- Phenanthrolines/chemical synthesis*
- Phenanthrolines/chemistry
- Photons
- Ruthenium/chemistry*
- Zebrafish
- PubMed
- 24166837 Full text @ Chemistry
A new dinuclear RuII polypyridyl complex, [(bpy)2Ru(H2bpip)Ru(bpy)2]4+ (RuH2bpip, bpy=2,2-bipyridine, H2bpip=2,6-pyridyl(imidazo[4,5-f][1,10]phenanthroline), was developed to act as a one- and two-photon luminescent probe for biological Cu2+ detection. This RuII complex shows a significant two-photon absorption cross section (400GM) and displays a remarkable one- and two-photon luminescence switch in the presence of Cu2+ ions. Importantly, RuH2bpip can selectively recognise Cu2+ in aqueous media in the presence of other abundant cellular cations (such as Na+, K+, Mg2+, and Ca2+), trace metal ions in organisms (such as Zn2+, Ag+, Fe3+, Fe2+, Ni2+, Mn2+, and Co2+), prevalent toxic metal ions in the environment (such as Cd2+, Hg2+, and Cr3+), and amino acids, with high sensitivity (detection limitd3.33×108 M) and a rapid response time (d15s). The biological applications of RuH2bpip were also evaluated and it was found to exhibit low cytotoxicity, good water solubility, and membrane permeability; RuH2bpip was, therefore, employed as a sensing probe for the detection of Cu2+ in living cells and zebrafish.