PUBLICATION

Direct and indirect roles of Fgf3 and Fgf10 in innervation and vascularisation of the vertebrate hypothalamic neurohypophysis

Authors
Liu, F., Pogoda, H.M., Pearson, C.A., Ohyama, K., Löhr, H., Hammerschmidt, M., and Placzek, M.
ID
ZDB-PUB-130222-26
Date
2013
Source
Development (Cambridge, England)   140(5): 1111-1122 (Journal)
Registered Authors
Hammerschmidt, Matthias, Löhr, Heiko, Pogoda, Hans-Martin
Keywords
FGF, guidance, hypothalamus, zebrafish, chick
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Axons/metabolism
  • Axons/physiology
  • Cells, Cultured
  • Chick Embryo/blood supply
  • Chick Embryo/innervation
  • Chick Embryo/metabolism
  • Embryo, Nonmammalian/blood supply
  • Embryo, Nonmammalian/innervation
  • Embryo, Nonmammalian/metabolism
  • Fibroblast Growth Factor 10/genetics
  • Fibroblast Growth Factor 10/metabolism
  • Fibroblast Growth Factor 10/physiology*
  • Fibroblast Growth Factor 3/genetics
  • Fibroblast Growth Factor 3/metabolism
  • Fibroblast Growth Factor 3/physiology*
  • Hypothalamo-Hypophyseal System/blood supply
  • Hypothalamo-Hypophyseal System/embryology
  • Hypothalamo-Hypophyseal System/metabolism
  • Models, Biological
  • Neovascularization, Physiologic/genetics*
  • Neovascularization, Physiologic/physiology
  • Pituitary Gland, Posterior/blood supply*
  • Pituitary Gland, Posterior/embryology
  • Pituitary Gland, Posterior/innervation*
  • Vertebrates/embryology
  • Vertebrates/genetics
  • Vertebrates/metabolism
  • Zebrafish/embryology
  • Zebrafish/genetics
  • Zebrafish/physiology
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
  • Zebrafish Proteins/physiology*
(all 35)
PubMed
23404108 Full text @ Development
Abstract

The neurohypophysis is a crucial component of the hypothalamo-pituitary axis, serving as the site of release of hypothalamic neurohormones into a plexus of hypophyseal capillaries. The growth of hypothalamic axons and capillaries to the forming neurohypophysis in embryogenesis is therefore crucial to future adult homeostasis. Using ex vivo analyses in chick and in vivo analyses in mutant and transgenic zebrafish, we show that Fgf10 and Fgf3 secreted from the forming neurohypophysis exert direct guidance effects on hypothalamic neurosecretory axons. Simultaneously, they promote hypophyseal vascularisation, exerting early direct effects on endothelial cells that are subsequently complemented by indirect effects. Together, our studies suggest a model for the integrated neurohemal wiring of the hypothalamo-neurohypophyseal axis.

Genes / Markers
Marker Marker Type Name
avpGENEarginine vasopressin
crabp1aGENEcellular retinoic acid binding protein 1a
drd2aGENEdopamine receptor D2a
drd2bGENEdopamine receptor D2b
drd2lGENEdopamine receptor D2 like
fgf3GENEfibroblast growth factor 3
fgfr1aGENEfibroblast growth factor receptor 1a
pomcaGENEproopiomelanocortin a
prlGENEprolactin
thGENEtyrosine hydroxylase
1 - 10 of 10
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Figures
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Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
fr31TgTransgenic Insertion
    pd1TgTransgenic Insertion
      s896TgTransgenic Insertion
        t24152
          Point Mutation
          ubs4TgTransgenic Insertion
            zc49TgTransgenic Insertion
              zf44TgTransgenic Insertion
                zf113TgTransgenic Insertion
                  1 - 8 of 8
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                  Human Disease / Model
                  No data available
                  Sequence Targeting Reagents
                  No data available
                  Fish
                  Antibodies
                  Orthology
                  No data available
                  Engineered Foreign Genes
                  Marker Marker Type Name
                  EGFPEFGEGFP
                  GAL4FFEFGGAL4FF
                  GFPEFGGFP
                  KALTA4EFGKALTA4
                  mCherryEFGmCherry
                  RFPEFGRFP
                  1 - 6 of 6
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                  Mapping