PUBLICATION
Efficient genome editing in zebrafish using a CRISPR-Cas system
- Authors
- Hwang, W.Y., Fu, Y., Reyon, D., Maeder, M.L., Tsai, S.Q., Sander, J.D., Peterson, R.T., Yeh, J.R., and Joung, J.K.
- ID
- ZDB-PUB-130211-4
- Date
- 2013
- Source
- Nat. Biotechnol. 31(3): 227-229 (Journal)
- Registered Authors
- Peterson, Randall, Yeh, Jing-Ruey (Joanna)
- Keywords
- none
- MeSH Terms
-
- Animals
- Base Sequence
- DNA/genetics
- DNA Cleavage
- Embryo, Nonmammalian
- Endonucleases/genetics
- Genetic Engineering
- Genome*
- Inverted Repeat Sequences*
- Molecular Sequence Data
- Mutation
- Nucleic Acid Conformation
- RNA Editing
- RNA, Guide, Kinetoplastida/genetics*
- Zebrafish/genetics*
- PubMed
- 23360964 Full text @ Nat. Biotechnol.
Citation
Hwang, W.Y., Fu, Y., Reyon, D., Maeder, M.L., Tsai, S.Q., Sander, J.D., Peterson, R.T., Yeh, J.R., and Joung, J.K. (2013) Efficient genome editing in zebrafish using a CRISPR-Cas system. Nat. Biotechnol.. 31(3):227-229.
Abstract
In bacteria, foreign nucleic acids are silenced by clustered, regularly interspaced, short palindromic repeats (CRISPR)–CRISPR-associated (Cas) systems. Bacterial type II CRISPR systems have been adapted to create guide RNAs that direct site-specific DNA cleavage by the Cas9 endonuclease in cultured cells. Here we show that the CRISPR-Cas system functions in vivo to induce targeted genetic modifications in zebrafish embryos with efficiencies similar to those obtained using zinc finger nucleases and transcription activator–like effector nucleases.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping