PUBLICATION

The role of Nfil3 in zebrafish hematopoiesis

Authors
Progatzky, F., Taylor, H., Bugeon, L., Cassidy, S., Radbruch, A., Dallman, M.J., and Lamb, J.R.
ID
ZDB-PUB-120510-5
Date
2012
Source
Developmental and comparative immunology   38(1): 187-192 (Journal)
Registered Authors
Bugeon, Laurence, Dallman, Maggie, Lamb, Jonathan, Progatzky, Fränze
Keywords
transcription factor, Nfil3, myelopoiesis, zebrafish, eosinophilis
MeSH Terms
  • Animals
  • Embryo, Nonmammalian/metabolism
  • Morpholinos/metabolism
  • Myeloid Cells/metabolism
  • Myelopoiesis*
  • Transcription Factors/genetics
  • Transcription Factors/metabolism*
  • Zebrafish/embryology*
  • Zebrafish/metabolism*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed
22561072 Full text @ Dev. Comp. Immunol.
Abstract

Nfil3, a transcription factor that has an array of functions in immune cells, has been described as key regulator of CD8α+ dendritic cell and natural killer cell development in mice. In this report we show that Nfil3 is enriched in the myeloid compartment of adult zebrafish including eosinophils. Knockdown of Nfil3 in pu.1:GFP embryos resulted in a reduced number of myeloid cells as early as 24 h post-fertilization, while erythropoiesis was unaffected. Using mpx and fms-fluorescent transgenic fish we found that all myeloid cell lineages, and in particular macrophages, had reduced numbers at 4 days post-fertilization. This was reflected by less myeloid cells accumulating at a wound site. Pu.1, l-plastin, csf1r and mpx had reduced expression in Nfil3 morphants while runx1, gata1 and rag1 were unaffected. Collectively, these results describe a conserved expression pattern of Nfil3 in evolutionarily divergent species and indicate that Nfil3 is central to myeloid lineage commitment.

Genes / Markers
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Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
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Mapping