PUBLICATION

Spatiotemporal resolution of the Ntla transcriptome in axial mesoderm development

Authors
Shestopalov, I.A., Pitt, C.L., and Chen, J.K.
ID
ZDB-PUB-120202-6
Date
2012
Source
Nature Chemical Biology   8(3): 270-276 (Journal)
Registered Authors
Chen, James K., Shestopalov, Ilya
Keywords
none
Datasets
GEO:GSE31882, GEO:GSE31881, GEO:GSE31880
MeSH Terms
  • Animals
  • Fetal Proteins
  • Gene Expression Regulation, Developmental*
  • Mesoderm/embryology*
  • Mesoderm/metabolism*
  • T-Box Domain Proteins/genetics*
  • Time Factors
  • Transcriptome*
  • Zebrafish/embryology*
  • Zebrafish/genetics*
  • Zebrafish Proteins/genetics*
PubMed
22286130 Full text @ Nat. Chem. Biol.
Abstract
Transcription factors have diverse roles during embryonic development, combinatorially controlling cellular states in a spatially and temporally defined manner. Resolving the dynamic transcriptional profiles that underlie these patterning processes is essential for understanding embryogenesis at the molecular level. Here we show how temporal, tissue-specific changes in embryonic transcription factor function can be discerned by integrating caged morpholino oligonucleotides with photoactivatable fluorophores, fluorescence-activated cell sorting and microarray technologies. As a proof of principle, we have dynamically profiled No tail a (Ntla)-dependent genes at different stages of axial mesoderm development in zebrafish, discovering discrete sets of transcripts that are coincident with either notochord cell fate commitment or differentiation. Our studies reveal new regulators of notochord development and the sequential activation of distinct transcriptomes within a cell lineage by a single transcriptional factor and demonstrate how optically controlled chemical tools can dissect developmental processes with spatiotemporal precision.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping