PUBLICATION

Lhx2 and lhx9 determine neuronal differentiation and compartition in the caudal forebrain by regulating wnt signaling

Authors
Peukert, D., Weber, S., Lumsden, A., and Scholpp, S.
ID
ZDB-PUB-120105-73
Date
2011
Source
PLoS Biology   9(12): e1001218 (Journal)
Registered Authors
Lumsden, Andrew, Peukert, Daniela, Scholpp, Steffen, Weber, Sabrina
Keywords
Embryos, Thalamus, Wnt signaling cascade, Neuronal differentiation, Zebrafish, Neurons, Diencephalon, Electroporation
MeSH Terms
  • Animals
  • Body Patterning/genetics*
  • Cadherins/physiology
  • Gene Expression Regulation, Developmental
  • LIM-Homeodomain Proteins/deficiency
  • LIM-Homeodomain Proteins/genetics
  • LIM-Homeodomain Proteins/physiology*
  • Nerve Tissue Proteins/deficiency
  • Nerve Tissue Proteins/genetics
  • Nerve Tissue Proteins/physiology*
  • Neural Tube/physiology
  • Neurogenesis/physiology*
  • Prosencephalon/embryology*
  • Signal Transduction/physiology
  • Thalamus/embryology
  • Transcription Factors/deficiency
  • Transcription Factors/genetics
  • Transcription Factors/physiology*
  • Wnt Proteins/physiology*
  • Zebrafish
  • Zebrafish Proteins/deficiency
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/physiology*
(all 23)
PubMed
22180728 Full text @ PLoS Biol.
Abstract

Initial axial patterning of the neural tube into forebrain, midbrain, and hindbrain primordia occurs during gastrulation. After this patterning phase, further diversification within the brain is thought to proceed largely independently in the different primordia. However, mechanisms that maintain the demarcation of brain subdivisions at later stages are poorly understood. In the alar plate of the caudal forebrain there are two principal units, the thalamus and the pretectum, each of which is a developmental compartment. Here we show that proper neuronal differentiation of the thalamus requires Lhx2 and Lhx9 function. In Lhx2/Lhx9-deficient zebrafish embryos the differentiation process is blocked and the dorsally adjacent Wnt positive epithalamus expands into the thalamus. This leads to an upregulation of Wnt signaling in the caudal forebrain. Lack of Lhx2/Lhx9 function as well as increased Wnt signaling alter the expression of the thalamus specific cell adhesion factor pcdh10b and lead subsequently to a striking anterior-posterior disorganization of the caudal forebrain. We therefore suggest that after initial neural tube patterning, neurogenesis within a brain compartment influences the integrity of the neuronal progenitor pool and border formation of a neuromeric compartment.

Genes / Markers
Marker Marker Type Name
axin1GENEaxin 1
axin2GENEaxin 2 (conductin, axil)
dkk1bGENEdickkopf WNT signaling pathway inhibitor 1b
dlaGENEdeltaA
gad1bGENEglutamate decarboxylase 1b
gbx2GENEgastrulation brain homeobox 2
gsx1GENEGS homeobox 1
heltGENEhelt bHLH transcription factor
id2aGENEinhibitor of DNA binding 2a
lef1GENElymphoid enhancer-binding factor 1
1 - 10 of 20
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Figures
Figure Gallery (11 images) / 2
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Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
b700
    Small Deletion
    ia4TgTransgenic Insertion
      knu3TgTransgenic Insertion
        sb3TgTransgenic Insertion
          sud1TgTransgenic Insertion
            tm213
              Point Mutation
              tv42z
                Point Mutation
                w32TgTransgenic Insertion
                  zf255TgTransgenic Insertion
                    zf277EtTransgenic Insertion
                      1 - 10 of 11
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                      Human Disease / Model
                      No data available
                      Sequence Targeting Reagents
                      Target Reagent Reagent Type
                      lhx2bMO3-lhx2bMRPHLNO
                      lhx9MO1-lhx9MRPHLNO
                      pcdh10bMO1-pcdh10bMRPHLNO
                      wnt3aMO2-wnt3aMRPHLNO
                      1 - 4 of 4
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                      Fish
                      Antibodies
                      Orthology
                      Engineered Foreign Genes
                      Marker Marker Type Name
                      DsRedEFGDsRed
                      EGFPEFGEGFP
                      GFPEFGGFP
                      KALTA4EFGKALTA4
                      mCherryEFGmCherry
                      RFPEFGRFP
                      1 - 6 of 6
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                      Mapping