Zebrafish Stromal Cells have Endothelial Properties and Support Hematopoietic Cells
- Authors
- Lund, T.C., Glass, T.J., Somani, A., Nair, S., Tolar, J., Nyquist, M., Patrinostro, X., and Blazar, B.R.
- ID
- ZDB-PUB-110920-33
- Date
- 2012
- Source
- Experimental hematology 40(1): 61-70.e1 (Journal)
- Registered Authors
- Lund, Troy
- Keywords
- endothelial cells, mesenchymal stromal cell, zebrafish, stroma, hematopoiesis
- MeSH Terms
-
- Animals
- Cell Communication
- Cells, Cultured
- Coculture Techniques
- Endothelial Cells/cytology*
- Hematopoiesis
- Hematopoietic Stem Cells/cytology*
- Stromal Cells/cytology*
- Zebrafish
- PubMed
- 21920471 Full text @ Exp. Hematol.
The goal of this study was to determine if we could establish a mesenchymal stromal line from zebrafish that would support hematopoietic cells. Such a co-culture system would be a great benefit to study the hematopoietic cell-stromal cell interaction in both the in vitro and in vivo environments. Zebrafish stromal cells, ZStrC, were isolated from the “mesenchymal” tissue of the caudal tail and expanded in a specialized growth media. ZStrC were evaluated for phenotype, gene expression, and the ability to maintain zebrafish marrow cells in co-culture experiments. ZStrC showed mesenchymal and endothelial gene expression. Although ZStrC lacked the ability to differentiate into classic MSC lineages (osteocytes, adipocytes, chondrocytes), they did have the capacity for endotube formation on matrigel and LDL-uptake. ZStrC supported marrow cells for greater than 2 weeks in vitro. Importantly, the marrow cells were shown to retain homing ability in adoptive transfer experiments. ZStrC also were shown to improve hematopoietic recovery after sub-lethal irradiation after adoptive transfer. As the zebrafish model grows in popularity and importance in the study of hematopoiesis, new tools to aid in our understanding of the hematopoietic cell-stromal cell interaction are required. ZStrC represent an additional tool in the study of hematopoiesis and will be useful to understand the factors that mediate the stromal cell-hematopoietic cell interaction that are important in hematopoietic maintenance.