PUBLICATION
Identification of Z-OTU protein during zebrafish oogenesis and early embryogenesis
- Authors
- Yue, M.J., Mo, S.J., Song, P., and Gong, Y.Z.
- ID
- ZDB-PUB-110816-31
- Date
- 2011
- Source
- Dong wu xue yan jiu = Zoological research 32(4): 386-390 (Journal)
- Registered Authors
- Song, Ping
- Keywords
- danio rerio, OTU-related protease, DUB, Z-OTU protein, oogenesis, embryogenesis
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Cysteine Endopeptidases/chemistry
- Cysteine Endopeptidases/genetics
- Cysteine Endopeptidases/metabolism*
- Embryonic Development*
- Female
- Male
- Molecular Sequence Data
- Oocytes/cytology
- Oocytes/metabolism*
- Oogenesis*
- Protein Transport
- Rabbits
- Sequence Alignment
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish/metabolism*
- Zebrafish Proteins/chemistry
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 21842534 Full text @ Zool. Res.
Citation
Yue, M.J., Mo, S.J., Song, P., and Gong, Y.Z. (2011) Identification of Z-OTU protein during zebrafish oogenesis and early embryogenesis. Dong wu xue yan jiu = Zoological research. 32(4):386-390.
Abstract
Zebrafish (Danio rerio) Z-OTU, containing OTU and TUDOR domains, was predicted to be a member of OTU-related protease, a family of the deubiquitylating enzymes (DUBs). A previous report from our laboratory clearly describes the expression patterns of z-otu mRNA. Here, we characterized the Z-OTU protein during zebrafish oogenesis and early embryogenesis. After prokaryotic expression, the recombinant protein of the OTU domain and GST was purified and injected into rabbits to obtain the polyclonal antibody-anti-Z-OTU, which was used for immunohistochemistry in zebrafish ovaries and embryos. Interestingly, obvious differences existed between the expression patterns of z-otu mRNA and its protein during oogenesis and early embryogenesis. In stage I oocytes, z-otu mRNA was detected in cytoplasm while its protein existed in the germinal vesicle. In addition, its protein was distributed during entire oogenesis, while mRNA was not detected in oocytes at stage IV or mature oocytes. The z-otu mRNA disappeared after midblastula transition (MBT) and its protein gradually decreased after this stage. We inferred that Z-OTU protein, like other OTU-related protease with DUB activity, was required for germinal vesicle breakdown of oocytes during meiosis, germinal vesicle migration, and embryo cleavage maintenance.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping