PUBLICATION
miR-196 regulates axial patterning and pectoral appendage initiation
- Authors
- He, X., Yan, Y.L., Eberhart, J.K., Herpin, A., Wagner, T.U., Schartl, M., and Postlethwait, J.H.
- ID
- ZDB-PUB-110803-10
- Date
- 2011
- Source
- Developmental Biology 357(2): 463-77 (Journal)
- Registered Authors
- Eberhart, Johann, He, Xinjun, Postlethwait, John H., Schartl, Manfred, Wagner, Toni, Yan, Yi-Lin
- Keywords
- microRNA (miRNA), Mir196 (miR-196), axial skeletal patterning, pectoral fin, pharyngeal arch, retinoic acid
- MeSH Terms
-
- Animal Fins/embryology*
- Animal Fins/metabolism
- Animals
- Base Sequence
- Body Patterning/genetics*
- Bone and Bones/embryology
- Bone and Bones/metabolism
- Branchial Region/embryology
- Branchial Region/metabolism
- Embryo, Nonmammalian/metabolism
- Gene Expression Regulation, Developmental
- Genomics
- Homeodomain Proteins/genetics
- Homeodomain Proteins/metabolism
- MicroRNAs/genetics
- MicroRNAs/metabolism*
- Molecular Sequence Data
- Signal Transduction/genetics
- Tretinoin/metabolism
- Zebrafish/embryology*
- Zebrafish/genetics*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 21787766 Full text @ Dev. Biol.
Citation
He, X., Yan, Y.L., Eberhart, J.K., Herpin, A., Wagner, T.U., Schartl, M., and Postlethwait, J.H. (2011) miR-196 regulates axial patterning and pectoral appendage initiation. Developmental Biology. 357(2):463-77.
Abstract
Vertebrate Hox clusters contain protein-coding genes that regulate body axis development and microRNA (miRNA) genes whose functions are not yet well understood. We overexpressed the Hox cluster microRNA miR-196 in zebrafish embryos and found four specific, viable phenotypes: failure of pectoral fin bud initiation, deletion of the 6th pharyngeal arch, homeotic aberration and loss of rostral vertebrae, and reduced number of ribs and somites. Reciprocally, miR-196 knockdown evoked an extra pharyngeal arch, extra ribs, and extra somites, confirming endogenous roles of miR-196. miR-196 injection altered expression of hox genes and the signaling of retinoic acid through the retinoic acid receptor gene rarab. Knocking down rarab mimicked the pectoral fin phenotype of miR-196 overexpression, and reporter constructs tested in tissue culture and in embryos showed that the rarab 32UTR is a miR-196 target for pectoral fin bud initiation. These results show that a Hox cluster microRNA modulates development of axial patterning similar to nearby protein-coding Hox genes, and acts on appendicular patterning at least in part by modulating retinoic acid signaling.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping