PUBLICATION
Stimulatory effect of intracerebroventricular administration of orexin A on food intake in the zebrafish, Danio rerio
- Authors
- Yokobori, E., Kojima, K., Azuma, M., Kang, K.S., Maejima, S., Uchiyama, M., and Matsuda, K.
- ID
- ZDB-PUB-110609-3
- Date
- 2011
- Source
- Peptides 32(7): 1357-62 (Journal)
- Registered Authors
- Matsuda, Kouhei
- Keywords
- zebrafish, orexin A, food intake, orexigenic action, SB334867, orexin precursor mRNA
- MeSH Terms
-
- Animals
- Benzoxazoles/pharmacology
- Eating/drug effects*
- Eating/physiology
- Fasting
- Feeding Behavior/drug effects*
- Feeding Behavior/physiology
- Hypothalamus/cytology
- Hypothalamus/metabolism*
- Injections, Intraventricular
- Intracellular Signaling Peptides and Proteins*/metabolism
- Intracellular Signaling Peptides and Proteins*/pharmacology
- Neuropeptides*/metabolism
- Neuropeptides*/pharmacology
- Orexin Receptors
- Orexins
- RNA, Messenger/analysis
- Receptors, G-Protein-Coupled/antagonists & inhibitors*
- Receptors, G-Protein-Coupled/metabolism
- Receptors, Neuropeptide/antagonists & inhibitors*
- Receptors, Neuropeptide/metabolism
- Reverse Transcriptase Polymerase Chain Reaction
- Urea/analogs & derivatives
- Urea/pharmacology
- Zebrafish/physiology*
- PubMed
- 21616109 Full text @ Peptides
Citation
Yokobori, E., Kojima, K., Azuma, M., Kang, K.S., Maejima, S., Uchiyama, M., and Matsuda, K. (2011) Stimulatory effect of intracerebroventricular administration of orexin A on food intake in the zebrafish, Danio rerio. Peptides. 32(7):1357-62.
Abstract
Orexin is a potent orexigenic neuropeptide implicated in feeding regulation of mammals. However, except for the case of goldfish, the involvement of orexin in the feeding behavior of teleost fish has not well been studied. Therefore, we investigated the role of orexin on food intake using a zebrafish (Danio rerio) model. We examined the effect of feeding status on orexin-like immunoreactivity and the expression level of orexin transcript in the brain. The number of neuronal cells showing orexin-like immunoreactivity in the hypothalamic region, including the posterior tuberal nucleus, was significantly increased in fish fasted for 7 days. Orexin precursor mRNA levels in the brain obtained from fish fasted for 7 days were higher than those in fish that had been fed normally. We then investigated the effect of intracerebroventricular (ICV) administration of orexin A on food intake. Cumulative food intake was significantly increased by ICV administration of orexin A (at 0.3 and 3 pmol/g body weight, BW) during a 60-min observation period after treatment. The orexin A-induced orexigenic action (at 0.3 pmol/g BW) was blocked by treatment with an orexin receptor antagonist, SB334867, at 10 pmol/g BW. These results indicate that orexin A acts as feeding regulator in the zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping