PUBLICATION
Acute Restraint Stress in Zebrafish: Behavioral Parameters and Purinergic Signaling
- Authors
- Piato, A.L., Rosemberg, D.B., Capiotti, K.M., Siebel, A.M., Herrmann, A.P., Ghisleni, G., Vianna, M.R., Bogo, M.R., Lara, D.R., and Bonan, C.D.
- ID
- ZDB-PUB-110603-6
- Date
- 2011
- Source
- Neurochemical research 36(10): 1876-86 (Journal)
- Registered Authors
- Bonan, Carla Denise, Vianna, Mônica Ryff Moreira Roca
- Keywords
- zebrafish, acute restraint stress (ARS), behavior, memory, nucleotide hydrolysis, adenosine
- MeSH Terms
-
- Adenosine Deaminase/genetics
- Adenosine Deaminase/metabolism
- Adenosine Triphosphatases/genetics
- Adenosine Triphosphatases/metabolism
- Adenosine Triphosphate/metabolism
- Animals
- Behavior, Animal/physiology*
- Male
- Memory/physiology
- Motor Activity/physiology
- Purines/metabolism*
- Restraint, Physical*
- Signal Transduction/physiology*
- Stress, Psychological*
- Zebrafish/physiology*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- PubMed
- 21603935 Full text @ Neurochem. Res.
Citation
Piato, A.L., Rosemberg, D.B., Capiotti, K.M., Siebel, A.M., Herrmann, A.P., Ghisleni, G., Vianna, M.R., Bogo, M.R., Lara, D.R., and Bonan, C.D. (2011) Acute Restraint Stress in Zebrafish: Behavioral Parameters and Purinergic Signaling. Neurochemical research. 36(10):1876-86.
Abstract
Despite the extensive knowledge about the effects of acute restraint stress (ARS) in rodents, zebrafish research is still elementary in this field, and the consequences of stress on purinergic system are unclear. Therefore, we evaluated the effects of ARS on behavior, biochemical, and molecular parameters in zebrafish brain. Animals were submitted to a 90 min ARS protocol and tested for anxiety levels, exploratory behavior, and memory performance. Furthermore, we analyzed ectonucleotidase and adenosine deaminase activities and their gene expression profile, as well as transcription of adenosine receptors. ARS increased anxiety, but did not impair locomotion or cognition. ARS significantly increased ATP hydrolysis, decreased cytosolic ADA activity, and changed the entpd and adora gene expression. In conclusion, ARS disturbed zebrafish behavior, and we hypothesize that the augmentation in adenosine-mediated signaling may be a strategy to reestablish homeostasis and normal behavior after a stressful event.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping