PUBLICATION
Rack1 is required for Vangl2 membrane localization and planar cell polarity signaling while attenuating canonical Wnt activity
- Authors
- Li, S., Esterberg, R., Lachance, V., Ren, D., Radde-Gallwitz, K., Chi, F., Parent, J.L., Fritz, A., and Chen, P.
- ID
- ZDB-PUB-110131-13
- Date
- 2011
- Source
- Proceedings of the National Academy of Sciences of the United States of America 108(6): 2264-2269 (Journal)
- Registered Authors
- Fritz, Andreas
- Keywords
- none
- MeSH Terms
-
- Animals
- Cell Division/physiology
- Cell Membrane/genetics
- Cell Membrane/metabolism
- Cell Polarity/physiology*
- Gastrula/cytology
- Gastrula/metabolism*
- Gastrulation/physiology*
- Membrane Proteins/genetics
- Membrane Proteins/metabolism*
- Mice
- Protein Structure, Tertiary
- Protein Transport/physiology
- Receptors, Cell Surface/genetics
- Receptors, Cell Surface/metabolism*
- Signal Transduction/physiology*
- Wnt Proteins/genetics
- Wnt Proteins/metabolism*
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 21262816 Full text @ Proc. Natl. Acad. Sci. USA
Citation
Li, S., Esterberg, R., Lachance, V., Ren, D., Radde-Gallwitz, K., Chi, F., Parent, J.L., Fritz, A., and Chen, P. (2011) Rack1 is required for Vangl2 membrane localization and planar cell polarity signaling while attenuating canonical Wnt activity. Proceedings of the National Academy of Sciences of the United States of America. 108(6):2264-2269.
Abstract
The vertebrate planar cell polarity (PCP) pathway shares molecular components with the β-catenin-mediated canonical Wnt pathway but acts through membrane complexes containing Vang or Frizzled to orient neighboring cells coordinately. The molecular interactions underlying the action of Vang in PCP signaling and specification, however, are yet to be delineated. Here, we report the identification of Rack1 as an interacting protein of a vertebrate Vang protein, Vangl2. We demonstrate that Rack1 is required in zebrafish for PCP-regulated processes, including oriented cell division, cellular polarization, and convergent extension during gastrulation. We further show that the knockdown of Rack1 affects membrane localization of Vangl2 and that the Vangl2-interacting domain of Rack1 has a dominant-negative effect on Vangl2 localization and gastrulation. Moreover, Rack1 antagonizes canonical Wnt signaling. Together, our data suggest that Rack1 regulates the localization of an essential PCP protein and acts as a molecular switch to promote PCP signaling.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping