PUBLICATION
Discovery of an unusual alternative splicing pathway of the immunoglobulin heavy chain in a teleost fish, Danio rerio
- Authors
- Hu, Y.L., Zhu, L.Y., Xiang, L.X., and Shao, J.Z.
- ID
- ZDB-PUB-101108-5
- Date
- 2011
- Source
- Developmental and comparative immunology 35(3): 253-257 (Journal)
- Registered Authors
- Keywords
- Zebrafish, Immunoglobulin, mIgM-2
- MeSH Terms
-
- Alternative Splicing
- Amino Acid Sequence
- Animals
- Base Sequence
- Immunoglobulin Heavy Chains/chemistry
- Immunoglobulin Heavy Chains/genetics*
- Immunoglobulin Heavy Chains/metabolism*
- Immunoglobulin M/chemistry
- Immunoglobulin M/genetics
- Immunoglobulin M/metabolism
- Molecular Sequence Data
- Phylogeny
- Reverse Transcriptase Polymerase Chain Reaction
- Sequence Alignment
- Zebrafish/genetics*
- Zebrafish/immunology*
- Zebrafish/metabolism
- Zebrafish Proteins/chemistry
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/metabolism*
- PubMed
- 21035505 Full text @ Dev. Comp. Immunol.
Citation
Hu, Y.L., Zhu, L.Y., Xiang, L.X., and Shao, J.Z. (2011) Discovery of an unusual alternative splicing pathway of the immunoglobulin heavy chain in a teleost fish, Danio rerio. Developmental and comparative immunology. 35(3):253-257.
Abstract
In present study, we identified a novel membrane immunoglobulin M isotype from zebrafish (Danio rerio), which was designated as mIgM-2, adding a new member to the Immunoglobulin family in teleost fish. The full length of cloned mIgM-2 cDNA was 611bp, encoding 150 amino acids. The putative mIgM-2 protein sequence consists of one constant region and a trans-membrane region. Phylogenetic analysis showed that mIgM-2 grouped with the known zebrafish IgM sequences. The mIgM-2 mRNA was widely expressed in immune-related tissues including heart, spleen, liver, intestine, kidney, gill, brain, skin and muscle. In vivo stimulation with LPS significantly up-regulates the expression of mIgM-2. Our results will add new insight into the immunoglobulin class diversity of teleost fish, and to better understand the evolutionary history of adaptive immunity from fish to mammals as a whole.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping