PUBLICATION
4D retrospective lineage tracing using SPIM for zebrafish organogenesis studies
- Authors
- Swoger, J., Muzzopappa, M., López-Schier, H., and Sharpe, J.
- ID
- ZDB-PUB-101011-49
- Date
- 2011
- Source
- Journal of biophotonics 4(1-2): 122-134 (Journal)
- Registered Authors
- Lopez-Schier, Hernan, Muzzopappa, Mariana
- Keywords
- selective plane illumination microscopy, SPIM, cell tracking, zebrafish, lateral line
- MeSH Terms
-
- Animals
- Cell Lineage*
- Cell Tracking
- Imaging, Three-Dimensional/methods*
- Microscopy/methods*
- Organogenesis*
- Rotation
- Time Factors
- Zebrafish/embryology*
- PubMed
- 20925108 Full text @ J. Biophotonics
Citation
Swoger, J., Muzzopappa, M., López-Schier, H., and Sharpe, J. (2011) 4D retrospective lineage tracing using SPIM for zebrafish organogenesis studies. Journal of biophotonics. 4(1-2):122-134.
Abstract
A study demonstrating an imaging framework that permits the determination of cell lineages during organogenesis of the posterior lateral line in zebrafish is presented. The combination of Selective Plane Illumination Microscopy and specific fluorescent markers allows retrospective tracking of hair cell progenitors, and hence the derivation of their lineages within the primodium. It is shown that, because of its superior signal-to-noise ratio and lower photo-damaged properties, SPIM can provide significantly higher-quality images than Spinning Disk Confocal technology. This allows accurate 4D lineage tracing for the hair cells over tens of hours of primordium migration and neuromast development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping