PUBLICATION

A study of somatolactin actions by ectopic expression in transgenic zebrafish larvae

Authors
Wan, G., and Chan, K.M.
ID
ZDB-PUB-100910-4
Date
2010
Source
Journal of molecular endocrinology   45(5): 301-315 (Journal)
Registered Authors
Keywords
none
MeSH Terms
  • Actins/genetics
  • Actins/metabolism
  • Animals
  • Animals, Genetically Modified
  • Blotting, Southern
  • Catalase/metabolism
  • Cloning, Molecular
  • Fatty Acid Synthases/chemistry
  • Fatty Acid Synthases/genetics
  • Fatty Acid Synthases/metabolism
  • Fish Proteins/genetics*
  • Fish Proteins/metabolism*
  • Gene Expression
  • Glutathione Transferase/genetics
  • Glutathione Transferase/metabolism
  • Glycoproteins/genetics*
  • Glycoproteins/metabolism*
  • Green Fluorescent Proteins/genetics
  • Insulin/genetics
  • Insulin/metabolism
  • Larva/genetics
  • Larva/metabolism
  • Leptin/genetics
  • Leptin/metabolism
  • Pituitary Hormones/genetics*
  • Pituitary Hormones/metabolism*
  • Pro-Opiomelanocortin/genetics
  • Pro-Opiomelanocortin/metabolism
  • Somatomedins/genetics
  • Somatomedins/metabolism
  • Sterol Regulatory Element Binding Protein 1/genetics
  • Sterol Regulatory Element Binding Protein 1/metabolism
  • Vitellogenins/chemistry
  • Vitellogenins/genetics
  • Vitellogenins/metabolism
  • Zebrafish*/genetics
  • Zebrafish*/growth & development
  • Zebrafish*/metabolism
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
(all 40)
PubMed
20801895 Full text @ J. Mol. Endocrinol.
Abstract
Somatolactin (SL) is a fish-specific hormone that belongs to the prolactin (PRL) and growth hormone (GH) family. Recently, two forms of SL, SLalpha and SLbeta, have been found in some species, and may have different actions and functions. To investigate the role of SL in fish growth and metabolism, we generated transgenic fish founders with ectopic expression of SLalpha and SLbeta to study the physiological functions and actions of these SLs among several marker genes. We fused the cDNAs encoding the precursor SLs in frame to a zebrafish beta-actin gene promoter to generate transgenic zebrafish lines that were coinjected with a GFP protein driven by the same promoter. The transgenic zebrafish were selected based on GFP expression and confirmed by genomic polymerase chain reaction (PCR), Southern blot analysis, and transgene expression. Investigations into the expression of marker genes in larvae on different pathways using real-time PCR have provided a general understanding of the actions of SLs. This study found that the overexpression of SLalpha and SLbeta in vivo significantly enhanced the transcription of insulin-like growth factors (IGFs), insulin, leptin, sterol regulatory element binding protein 1 (SREBP1) and fatty acid synthase (FAS), as well as the expression level of vitellogenin (VTG) and melanocyte-stimulating hormone (MSH), while causing reduced levels of catalase (CAT) and glutathione S-transferase (GST) in the larvae of transgenic zebrafish.
Genes / Markers
Marker Marker Type Name
acacbGENEacetyl-CoA carboxylase beta
actb1GENEactin, beta 1
catGENEcatalase
gh1GENEgrowth hormone 1
gstp1.2GENEglutathione S-transferase pi 1.2
igf1GENEinsulin-like growth factor 1
igf2aGENEinsulin-like growth factor 2a
igf2bGENEinsulin-like growth factor 2b
igf3GENEinsulin-like growth factor 3
insGENEpreproinsulin
1 - 10 of 19
Show
Figures
No images available
Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
zf239TgTransgenic Insertion
    zf240TgTransgenic Insertion
      zf241TgTransgenic Insertion
        1 - 3 of 3
        Show
        Human Disease / Model
        No data available
        Sequence Targeting Reagents
        No data available
        Fish
        Fish
        zf239Tg
        zf240Tg
        zf241Tg
        1 - 3 of 3
        Show
        Antibodies
        Orthology
        Engineered Foreign Genes
        Marker Marker Type Name
        GFPEFGGFP
        1 - 1 of 1
        Show
        Mapping
        Your Input Welcome
        We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate. We will review your comments promptly.
        Citation
        Wan, G., and Chan, K.M. (2010) A study of somatolactin actions by ectopic expression in transgenic zebrafish larvae. Journal of molecular endocrinology. 45(5):301-315.