PUBLICATION
Cloning, characterization, and expression analysis of Toll-like receptor-7 cDNA from common carp, Cyprinus carpio L
- Authors
- Tanekhy, M., Kono, T., and Sakai, M.
- ID
- ZDB-PUB-100820-13
- Date
- 2010
- Source
- Comparative biochemistry and physiology. Part D, Genomics & proteomics 5(4): 245-255 (Journal)
- Registered Authors
- Keywords
- Cyprinus carpio L., cDNA, TLR7, Sequence analysis
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Base Sequence
- Carps/classification
- Carps/genetics*
- Carps/metabolism
- Cloning, Molecular
- DNA, Complementary/genetics*
- Gills/metabolism
- Humans
- Oncorhynchus mykiss/genetics
- Oncorhynchus mykiss/metabolism
- Phylogeny
- RNA, Messenger/genetics
- RNA, Messenger/metabolism
- Reverse Transcriptase Polymerase Chain Reaction
- Sequence Alignment
- Takifugu/genetics
- Takifugu/metabolism
- Toll-Like Receptor 7/genetics*
- Toll-Like Receptor 7/metabolism*
- Zebrafish/genetics
- Zebrafish/metabolism
- PubMed
- 20709611 Full text @ Comp. Biochem. Physiol. D Genom. Prot.
Citation
Tanekhy, M., Kono, T., and Sakai, M. (2010) Cloning, characterization, and expression analysis of Toll-like receptor-7 cDNA from common carp, Cyprinus carpio L. Comparative biochemistry and physiology. Part D, Genomics & proteomics. 5(4):245-255.
Abstract
The Toll-like receptor 7 (TLR7) is activated by single strand RNA and RNA-like compounds (imidazoquinoline), and it induces interferon production. We identified and described carp TLR7 cDNA and its mRNA expression. The full-length cDNA of carp TLR7 gene is 3427bp, encoding 1049 amino acids (AB553573). The similarities of carp TLR7 with zebrafish, rainbow trout, fugu, and human TLR7 were 89.6, 83.4, 80.6 and 74.6%, respectively, at the amino acid sequence level. Furthermore, the expression of TLR7 mRNA was investigated in normal tissues of carp by semi-quantitative RT-PCR analysis. Carp TLR7 expression was exhibited in healthy tissues (kidney, brain, spleen, skin, intestine, muscle, liver, gills and heart) and though the expression level in each tissue varied among healthy fish. Carp TLR7 expression was significantly increased in head kidney stimulated with TLR7 agonist, imiquimod, at 8, 24 and 48h in vitro when compared to expression in the control group. Moreover, carp head kidney leukocytes produced elevated levels of pro-inflammatory and type 1 interferon cytokine mRNA in response to imiquimod stimulation.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping