PUBLICATION
Inhibition of the microRNA pathway in zebrafish by siRNA
- Authors
- Fjose, A., and Zhao, X.F.
- ID
- ZDB-PUB-100420-27
- Date
- 2010
- Source
- Methods in molecular biology (Clifton, N.J.) 629: 239-255 (Chapter)
- Registered Authors
- Fjose, Anders
- Keywords
- RNAi, siRNA, microRNA, miR-430, zebrafish
- MeSH Terms
-
- Animals
- MicroRNAs/antagonists & inhibitors*
- RNA Interference*
- RNA, Small Interfering/metabolism*
- Zebrafish/embryology
- Zebrafish/genetics*
- PubMed
- 20387153 Full text @ Meth. Mol. Biol.
Citation
Fjose, A., and Zhao, X.F. (2010) Inhibition of the microRNA pathway in zebrafish by siRNA. Methods in molecular biology (Clifton, N.J.). 629:239-255.
Abstract
The microRNA (miRNA) pathway and the phenomenon of RNA interference (RNAi), which have both been shown to involve targeting of mRNAs by small RNA molecules, are interconnected and depend partly on the same cellular machinery. RNAi in vertebrates was first reported in zebrafish (Danio rerio) 10 years ago. However, reliable RNAi-based gene silencing techniques, based on injection of small interfering RNAs (siRNAs) into zygotes, have not been established for this important vertebrate model because of unspecific developmental defects. We have recently shown that these side effects can be attributed to inhibition of the miRNA pathway by siRNAs at early embryonic stages. This review highlights these findings and the function of microRNAs in zebrafish development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping