PUBLICATION

KDM7 is a dual demethylase for histone H3 Lys 9 and Lys 27 and functions in brain development

Authors
Tsukada, Y., Ishitani, T., and Nakayama, K.I.
ID
ZDB-PUB-100309-10
Date
2010
Source
Genes & Development   24(5): 432-437 (Journal)
Registered Authors
Ishitani, Tohru
Keywords
Demethylase, JmjC, methylation, histone, chromatin, epigenetics
MeSH Terms
  • Animals
  • Brain/embryology*
  • Brain/enzymology*
  • Cells, Cultured
  • Embryo, Nonmammalian/embryology
  • Embryo, Nonmammalian/enzymology
  • Follistatin/metabolism
  • Gene Expression Regulation, Developmental
  • Histones/metabolism*
  • Jumonji Domain-Containing Histone Demethylases/genetics
  • Jumonji Domain-Containing Histone Demethylases/metabolism*
  • Mice
  • Neurons/metabolism
  • Zebrafish/embryology
  • Zebrafish Proteins/metabolism*
PubMed
20194436 Full text @ Genes & Dev.
Abstract
Methylation of histone H3 Lys 9 and Lys 27 (H3K9 and H3K27) is associated with transcriptional silencing. Here we show that KDM7, a JmjC domain-containing protein, catalyzes demethylation of both mono- or dimethylated H3K9 and H3K27. Inhibition of KDM7 orthologs in zebrafish resulted in developmental brain defects. KDM7 interacts with the follistatin gene locus, and KDM7 depletion in mammalian neuronal cells suppressed follistatin gene transcription in association with increased levels of dimethylated H3K9 and H3K27. Our findings identify KDM7 as a dual demethylase for H3K9 and H3K27 that functions as an eraser of silencing marks on chromatin during brain development.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping