PUBLICATION

Phosphorylation of Junb family proteins by the Jun N-terminal kinase supports tissue regeneration in zebrafish

Authors
Ishida, T., Nakajima, T., Kudo, A., and Kawakami, A.
ID
ZDB-PUB-100211-30
Date
2010
Source
Developmental Biology   340(2): 468-479 (Journal)
Registered Authors
Kawakami, Atsushi, Kudo, Akira
Keywords
Regeneration, Zebrafish, Finfold, Junb, Junb-like, JNK, Wound healing
MeSH Terms
  • Animals
  • In Situ Hybridization
  • JNK Mitogen-Activated Protein Kinases/metabolism*
  • Models, Biological
  • Phosphorylation
  • Proto-Oncogene Proteins c-jun/metabolism*
  • Regeneration/genetics
  • Zebrafish/genetics
  • Zebrafish/metabolism*
(all 9)
PubMed
20144602 Full text @ Dev. Biol.
Abstract
Tissue regeneration is fundamental for multi-cellular organisms to maintain their integrity, but the competence of tissue restoration is different depending on tissues, species, and ages. In spite of the recent progresses of the molecular basis of regeneration, little is known about its regulative processes. We previously identified the junb and junb-like (junbl) as transcripts induced in response to tissue injury in zebrafish. It has been demonstrated that the mammalian JunB is not phosphorylated by the Jun N-terminal kinase (JNK) due to the absence of target site. Here, we show that the zebrafish Junb proteins retain the target site and are phosphorylated by the JNK. Significantly, we found that the phosphorylated Junb proteins (pJunbs) are necessary for adult and larval tissue regeneration, suggesting that the regulation of Junb proteins by phosphorylation is one of the molecular bases for the higher regeneration ability in zebrafish. We also show that the prolonged expression of junbs and their protein phosphorylation by the JNK after the wound healing stage are the unique and necessary features for regeneration. Thus, our data suggest that the transcriptional and post-transcriptional controls of junbs and their protein products are the important regulative steps that enable tissue regeneration in zebrafish.
Genes / Markers
Marker Marker Type Name
dlx5aGENEdistal-less homeobox 5a
junGENEJun proto-oncogene, AP-1 transcription factor subunit
junbaGENEJunB proto-oncogene, AP-1 transcription factor subunit a
junbbGENEJunB proto-oncogene, AP-1 transcription factor subunit b
jundGENEJunD proto-oncogene, AP-1 transcription factor subunit
pcnaGENEproliferating cell nuclear antigen
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Figures
Figure Gallery (13 images) / 2
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Expression
Phenotype
Fish Conditions Stage Phenotype Figure
WT + MO1-junbaphysical alteration: anatomical structureLong-pec
WT + MO1-junba + MO2-junbbphysical alteration: anatomical structureLong-pec
WT + MO2-junbbphysical alteration: anatomical structureLong-pec
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Mutations / Transgenics
No data available
Human Disease / Model
No data available
Sequence Targeting Reagents
Target Reagent Reagent Type
junbaMO1-junbaMRPHLNO
junbbMO2-junbbMRPHLNO
1 - 2 of 2
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Fish
Fish
WT
WT + MO1-junba
WT + MO1-junba + MO2-junbb
WT + MO2-junbb
1 - 4 of 4
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Antibodies
Name Type Antigen Genes Isotypes Host Organism
Ab1-junpolyclonalRabbit
Ab2-ctnnb1polyclonalRabbit
1 - 2 of 2
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Orthology
No data available
Engineered Foreign Genes
No data available
Mapping
No data available
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Citation
Ishida, T., Nakajima, T., Kudo, A., and Kawakami, A. (2010) Phosphorylation of Junb family proteins by the Jun N-terminal kinase supports tissue regeneration in zebrafish. Developmental Biology. 340(2):468-479.