PUBLICATION
Role of Melanocortin Receptor Accessory Proteins in the Function of Zebrafish Melanocortin Receptor Type 2
- Authors
- Agulleiro, M.J., Roy, S., Sánchez, E., Puchol, S., Gallo-Payet, N., and Cerdá-Reverter, J.M.
- ID
- ZDB-PUB-100211-21
- Date
- 2010
- Source
- Molecular and Cellular Endocrinology 320(1-2): 145-152 (Journal)
- Registered Authors
- Cerdá-Reverter, José Miguel
- Keywords
- ACTH, MRAP, MC2R, HPA-axis, Stress, Fish
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Cell Line
- Cell Membrane/metabolism
- Cyclic AMP/biosynthesis
- Gene Expression Profiling
- Gene Expression Regulation
- Humans
- Molecular Sequence Data
- Phylogeny
- RNA, Messenger/genetics
- RNA, Messenger/metabolism
- Receptor, Melanocortin, Type 2/genetics
- Receptor, Melanocortin, Type 2/metabolism*
- Sequence Alignment
- Signal Transduction
- Zebrafish/genetics
- Zebrafish/metabolism*
- Zebrafish Proteins/chemistry
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 20138960 Full text @ Mol. Cell. Endocrinol.
Citation
Agulleiro, M.J., Roy, S., Sánchez, E., Puchol, S., Gallo-Payet, N., and Cerdá-Reverter, J.M. (2010) Role of Melanocortin Receptor Accessory Proteins in the Function of Zebrafish Melanocortin Receptor Type 2. Molecular and Cellular Endocrinology. 320(1-2):145-152.
Abstract
In this paper, we identify three different MRAPs in zebrafish, zfMRAP1, zfMRAP2a and zfMRAP2b, and demonstrate that zfMC2R is not functional in the absence of MRAP expression. ZfMRAP1 expression was restricted to adipose tissue and the anterior kidney whereas MRAP2a and MRAP2b were expressed in all the tissues tested. Quantification of surface receptor and immunofluorescence studies indicated that the receptor is unable to translocate to membrane in the absence of MRAP isoforms. MRAP1 and MRAP2b are localized in the plasma membrane in the absence of zfMC2R expression but MRAP2b is retained in perinuclear position. MRAP1 and MRAP2a displayed an equivalent translocation capacity to the membrane of zfMC2R but only zfMRAP1 expression led to intracellular cAMP increases after ACTH stimulation. ZfMRAP2b had no effect on zfMC2R activity but both zfMRAP2 isoforms enhanced the zfMRAP1-assited cAMP intracellular increase, suggesting an interaction between zfMRAP1 and zfMRAP2s when regulating zfMC2R activity.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping